Application | Comment | Organism |
---|---|---|
analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Rubus plicatus |
analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Fragaria x ananassa |
analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Ribes nigrum |
analysis | an HPLC method for the determination of phenylalanine ammonia-lyase, flavanone 3-hydroxylase and flavonol synthase enzyme activity is proposed. This method is based on the determination of the compounds produced and consumed on the enzymatic reaction in just one chromatographic analysis. Optimisation of the method consideres kinetic studies to establish the incubation time to perform the assay. The method is an approach to measure the activities of the three enzymes simultaneously increasing the rapidity, selectivity and sensitivity over other methods | Ribes rubrum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fragaria x ananassa | - |
- |
- |
Ribes nigrum | - |
- |
- |
Ribes rubrum | - |
- |
- |
Rubus plicatus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Rubus plicatus | - |
fruit | - |
Fragaria x ananassa | - |
fruit | - |
Ribes nigrum | - |
fruit | - |
Ribes rubrum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine | - |
Rubus plicatus | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | - |
Fragaria x ananassa | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | - |
Ribes nigrum | (E)-cinnamate + NH3 | - |
? | |
L-phenylalanine | - |
Ribes rubrum | (E)-cinnamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAL | - |
Rubus plicatus |
PAL | - |
Fragaria x ananassa |
PAL | - |
Ribes nigrum |
PAL | - |
Ribes rubrum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rubus plicatus |
30 | - |
assay at | Fragaria x ananassa |
30 | - |
assay at | Ribes nigrum |
30 | - |
assay at | Ribes rubrum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Rubus plicatus |
7.3 | - |
assay at | Fragaria x ananassa |
7.3 | - |
assay at | Ribes nigrum |
7.3 | - |
assay at | Ribes rubrum |