Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | protection of reduced enzyme by Cd2+ against inactivation | Pseudomonas sp. | |
EDTA | potent inhibitor of the reduced enzyme; reduced enzyme | Pseudomonas sp. | |
additional information | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pseudomonas sp. | |
6 | - |
L-histidine | oxidized enzyme, in the absence of added metal | Pseudomonas sp. | |
6.3 | - |
L-histidine | reduced enzyme in the presence of 0.01 M EDTA | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | stimulates oxidized and reduced enzyme | Pseudomonas sp. | |
Fe2+ | required for optimal activity | Pseudomonas sp. | |
Mn2+ | stimulates oxidized and reduced enzyme | Pseudomonas sp. | |
additional information | - |
Pseudomonas sp. | |
Zn2+ | required for optimal activity | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
ATCC 11,299b | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas sp. |
1.2 | - |
0.1 mM Ca2+, oxidized enzyme | Pseudomonas sp. |
2.3 | - |
0.1 mM Mn2+, oxidized enzyme | Pseudomonas sp. |
2.8 | - |
0.1 mM Fe2+, oxidized enzyme | Pseudomonas sp. |
4.3 | - |
0.1 mM Zn2+, oxidized enzyme | Pseudomonas sp. |
6.2 | - |
0.1 mM Ca2+, reduced enzyme | Pseudomonas sp. |
7 | - |
0.1 mM Fe2+ or 0.1 mM Zn2+, reduced enzyme | Pseudomonas sp. |
9.1 | - |
0.1 mM Mn2+, reduced enzyme | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-histidine | natural substrate | Pseudomonas sp. | urocanate + NH3 | - |
? |