Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor | Streptomyces venezuelae | the enzyme is involved in the biosynthesis of TDP-desosamine | dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | Q9ZGH1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor | the enzyme is involved in the biosynthesis of TDP-desosamine | Streptomyces venezuelae | dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor | - |
? | |
dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose + S-adenosyl-L-methionine + reduced acceptor | a mechanism is proposed involving direct elimination of ammonium concerted with proton transfer to the nucleofuge from the adjacent alpha-hydroxyalkyl radical | Streptomyces venezuelae | dTDP-3-dehydro-4,6-dideoxy-alpha-D-glucopyranose + NH3 + L-methionine + 5'-deoxyadenosine + acceptor | - |
? | |
TDP-4-amino-4-deoxy-D-fucose + S-adenosyl-L-methionine + reduced acceptor | TDP-4-amino-4-deoxy-D-fucose undergoes deamination alone with a specific activity that is 3fold reduced with respect to deamination of dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose. A mechanism is proposed involving direct elimination of ammonium concerted with proton transfer to the nucleofuge from the adjacent alpha-hydroxyalkyl radical | Streptomyces venezuelae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DesII | - |
Streptomyces venezuelae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
TDP-4-amino-4-deoxy-D-fucose | the observed kcat for DesII-catalyzed deamination at pH 8.0 was 3.4fold lower compared to that measured for deamination of the natural substrate dTDP-4-amino-4,6-dideoxy-alpha-D-glucopyranose with the same enzyme preparation | Streptomyces venezuelae |