Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequences | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethylene glycol | inactivates the EAL holoenzyme | Homo sapiens | |
methanol | inactivates the EAL holoenzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
(S)-2-aminopropanol | binding to apo-EAL at 25°C in buffered aqueous solution | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | included in cofactor B12 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-aminopropanol | building of a enzyme-coenzyme-substrate ternary complex, the steps are: radical pair separation, first hydrogen atom transfer, radical rearrangement, second hydrogen atom transfer, radical pair recombination, and product release/substrate binding | Homo sapiens | propanal + NH3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
EAL | - |
Homo sapiens |
ethanolamine ammonia-lyase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Comparison of the rates of steady-state turnover of coenzyme B12-dependent enzymes (kcat 10-100 1/s at 25°C) with the rate of cleavage of the cobalt-carbon bond of coenzyme B12 in solution reveals that the enzyme increases the cleavage rate more than 100000000000fold | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8.2 | broad pH-optimum | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | adenosylcobalamin | Homo sapiens |