Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.1.B2 extracted from

  • Myers, R.S.; Amaro, R.E.; Luthey-Schulten, Z.A.; Davisson, V.J.
    Reaction coupling through interdomain contacts in imidazole glycerol phosphate synthase (2005), Biochemistry, 44, 11974-11985.
    View publication on PubMed
Search for more literature for 4.3.1.B2

Protein Variants

Protein Variants Comment Organism
D359A 2300fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 4fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 7.5fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
K196A 0.43fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 1fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 3fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
K196A/D359A 2300fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 4fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 1fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
N13A 130fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 3fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 350fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae
Q397A 7.5fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: L-glutamine), 1fold decrease in kcat/Km of N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate (cosubstrate: NH4+), 18fold decrease in kcat/Km of L-glutamine Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00027
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A Saccharomyces cerevisiae
0.0013
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme N13A Saccharomyces cerevisiae
0.0036
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme Q397A Saccharomyces cerevisiae
0.005
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae
0.0055
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme D359A Saccharomyces cerevisiae
0.0141
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A/D359A Saccharomyces cerevisiae
0.026
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A/D359A Saccharomyces cerevisiae
0.03
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme Q397A Saccharomyces cerevisiae
0.034
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A Saccharomyces cerevisiae
0.055
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
0.071
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme N13A Saccharomyces cerevisiae
0.109
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme D359A Saccharomyces cerevisiae
0.49
-
 L-glutamine pH 8.0, 30°C, mutant enzyme K196A Saccharomyces cerevisiae
1 4 L-glutamine pH 8.0, 30°C, mutant enzyme D359A Saccharomyces cerevisiae
1.8
-
 L-glutamine pH 8.0, 30°C, wild-type enzyme Saccharomyces cerevisiae
2.3
-
 L-glutamine pH 8.0, 30°C, mutant enzyme K196A/D359A Saccharomyces cerevisiae
4.8
-
 L-glutamine pH 8.0, 30°C, mutant enzyme N13A Saccharomyces cerevisiae
7
-
 L-glutamine pH 8.0, 30°C, mutant enzyme Q397A Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P33734
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Saccharomyces cerevisiae L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
additional information the enzyme also shows glutaminase activity. The glutaminase activity of the glutaminase domain is tightly regulated by the acceptor substrate domain. In IGP synthase the glutaminase and N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate binding sites are separated by 30 A. Using kinetic analyses of site-specific mutants and molecular dynamic simulations, it is determined that an interdomain salt bridge in IGP synthase between D359 and K196 (approximately 16 A from the N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate binding site) plays a key role in mediating communication between the two active sites. This interdomain contact modulates the glutaminase loop containing the histidine and glutamic acid of the catalytic triad to control glutamine hydrolysis Saccharomyces cerevisiae ?
-
 ?
NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Saccharomyces cerevisiae D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Synonyms

Synonyms Comment Organism
IGP synthase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00029
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme D359A Saccharomyces cerevisiae
0.0059
-
 L-glutamine pH 8.0, 30°C, mutant enzyme D359A Saccharomyces cerevisiae
0.007
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A/D359A Saccharomyces cerevisiae
0.007
-
 L-glutamine pH 8.0, 30°C, mutant enzyme K196A/D359A Saccharomyces cerevisiae
0.01 1 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme N13A Saccharomyces cerevisiae
0.019
-
 L-glutamine pH 8.0, 30°C, mutant enzyme N13A Saccharomyces cerevisiae
0.11
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A/D359A Saccharomyces cerevisiae
0.35
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme N13A Saccharomyces cerevisiae
0.38
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme Q397A Saccharomyces cerevisiae
0.39
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme D359A Saccharomyces cerevisiae
0.46
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A Saccharomyces cerevisiae
0.56
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme Q397A Saccharomyces cerevisiae
0.6
-
 L-glutamine pH 8.0, 30°C, mutant enzyme K196A Saccharomyces cerevisiae
0.74
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A Saccharomyces cerevisiae
0.845
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
1.4
-
 L-glutamine pH 8.0, 30°C, mutant enzyme Q397A Saccharomyces cerevisiae
5.4
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae
6.9
-
 L-glutamine pH 8.0, 30°C, wild-type enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00042
-
 L-glutamine pH 8.0, 30°C, mutant enzyme D359A Saccharomyces cerevisiae
0.0032
-
 L-glutamine pH 8.0, 30°C, mutant enzyme K196A/D359A Saccharomyces cerevisiae
0.004
-
 L-glutamine pH 8.0, 30°C, mutant enzyme N13A Saccharomyces cerevisiae
0.016
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme Q397A Saccharomyces cerevisiae
0.21
-
 L-glutamine pH 8.0, 30°C, mutant enzyme Q397A Saccharomyces cerevisiae
0.51
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A/D359A Saccharomyces cerevisiae
0.52
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme D359A Saccharomyces cerevisiae
1.2
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme Q397A Saccharomyces cerevisiae
1.2
-
 L-glutamine pH 8.0, 30°C, mutant enzyme K196A Saccharomyces cerevisiae
3.6
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme D359A Saccharomyces cerevisiae
3.8
-
 L-glutamine pH 8.0, 30°C, wild-type enzyme Saccharomyces cerevisiae
3.8
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A/D359A Saccharomyces cerevisiae
4.9
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme N13A Saccharomyces cerevisiae
9
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme N13A Saccharomyces cerevisiae
14
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, mutant enzyme K196A Saccharomyces cerevisiae
15
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: NH4+, wild-type enzyme Saccharomyces cerevisiae
1200
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, wild-type enzyme Saccharomyces cerevisiae
2800
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 30°C, cosubstrate: L-glutamine, mutant enzyme K196A Saccharomyces cerevisiae