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Literature summary for 4.3.1.B2 extracted from

  • Liebold, C.; List, F.; Kalbitzer, H.R.; Sterner, R.; Brunner, E.
    The interaction of ammonia and xenon with the imidazole glycerol phosphate synthase from Thermotoga maritima as detected by NMR spectroscopy (2010), Protein Sci., 19, 1774-1782.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.1.B2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Protein Variants

Protein Variants Comment Organism
T78M the mutation does not impair substrate binding to the active site of HisF Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: L-glutamine, wild-type enzyme Thermotoga maritima
0.0028
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: L-glutamine, HisH/HisF holoenzyme with a T78M mutant subunit HisF Thermotoga maritima
0.0036
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: NH4+, wild-type enzyme Thermotoga maritima
0.0059
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: NH4+, HisH/HisF holoenzyme with a T78M mutant subunit HisF Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Thermotoga maritima L-glutamate + D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?
NH4+ + N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate
-
 Thermotoga maritima D-erythro-1-(imidazol-4-yl)glycerol phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: L-glutamine, wild-type enzyme Thermotoga maritima
1.2
-
 N1-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamido-1-beta-D-ribofuranosyl 5'-phosphate pH 8.0, 25°C, cosubstrate: NH4+, wild-type enzyme Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Thermotoga maritima