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Literature summary for 4.3.2.1 extracted from

  • Sampaleanu, L.M.; Yu, B.; Howell, P.L.
    Mutational analysis of duck delta 2 crystallin and the structure of an inactive mutant with bound substrate provide insight into the enzymatic mechanism of argininosuccinate lyase (2002), J. Biol. Chem., 277, 4166-4175.
    View publication on PubMed
Search for more literature for 4.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Anas platyrhynchos

Crystallization (Commentary)

Crystallization (Comment) Organism
S283A mutant, hanging-drop vapor diffusion at room temperatur, 9 mg/ml protein in 10 mM Tris-HCl, pH 7.5 and 1 mM EDTA is preincubated with 75 mM argininosuccinate, subsequently 0.005 ml drops of the solution are mixed with an equal ammount of precipitating solution consisting of 12% polyethylene glycol 2000 MME, 300 mM MgCl2, 100 mM HEPES, pH 7.4, crystals diffract to 1.96 A resolution Anas platyrhynchos

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Anas platyrhynchos
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(Nomega-L-arginino)succinate Anas platyrhynchos
-
 fumarate + L-arginine
-
 r

Organism

Organism UniProt Comment Textmining
Anas platyrhynchos
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged ASL Anas platyrhynchos

Source Tissue

Source Tissue Comment Organism Textmining
eye lens Anas platyrhynchos
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Nomega-L-arginino)succinate
-
 Anas platyrhynchos fumarate + L-arginine
-
 ?
(Nomega-L-arginino)succinate
-
 Anas platyrhynchos fumarate + L-arginine
-
 r

Synonyms

Synonyms Comment Organism
delta2 crystallin
-
 Anas platyrhynchos