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Literature summary for 4.3.2.10 extracted from

  • Lisi, G.; Currier, A.; Loria, J.
    Glutamine hydrolysis by imidazole glycerol phosphate synthase displays temperature dependent allosteric activation (2018), Front. Mol. Biosci., 5, 4 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.2.10

Activating Compound

Activating Compound Comment Organism Structure
N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide binding of the allosteric effector ligand stimulates millisecond timescale motions in the enzyme that enhance its catalytic function. The flexibility of the apo enzyme is nearly identical to that of its N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide activated state at 70°C, whereas conformational motions are considerably different between the two forms of the enzyme at room temperature. Allosteric activation by N'-[(5'-phosphoribulosyl)formimino]-5-aminoimidazole-4-carboxamide-ribonucleotide decreases to 65fold at 70°C, compared to 4200fold at 30°C Thermotoga maritima

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermotoga maritima
expression in Escherichia coli BL21(DE3) Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 L-glutamine pH 8.0, 30°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
1.32
-
 L-glutamine pH 8.0, 70°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
1.35
-
 L-glutamine pH 8.0, 40°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
1.36
-
 L-glutamine pH 8.0, 60°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
1.64
-
 L-glutamine pH 8.0, 50°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
2.96
-
 L-glutamine pH 8.0, 70°C Thermotoga maritima
2.96
-
 L-glutamine pH 8.0, 70°C, basal activity Thermotoga maritima
3.8
-
 L-glutamine pH 8.0, 50°C Thermotoga maritima
3.8
-
 L-glutamine pH 8.0, 50°C, basal activity Thermotoga maritima
3.89
-
 L-glutamine pH 8.0, 60°C Thermotoga maritima
3.89
-
 L-glutamine pH 8.0, 60°C, basal activity Thermotoga maritima
4.71
-
 L-glutamine pH 8.0, 40°C Thermotoga maritima
4.71
-
 L-glutamine pH 8.0, 40°C, basal activity Thermotoga maritima
4.91
-
 L-glutamine pH 8.0, 30°C Thermotoga maritima
4.91
-
 L-glutamine pH 8.0, 30°C, basal activity Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine Thermotoga maritima the enzyme is involved in histidine biosynthesis 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine Thermotoga maritima ATCC 43589 the enzyme is involved in histidine biosynthesis 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9X0C6 AND Q9X0C8 Q9X0C6: subunit HisF, Q9X0C8: subunit HisH
-
Thermotoga maritima Q9X0C8 and Q9X0C6 Q9X0C8: subunit HisH, Q9X0C6: subunit HisF
-
Thermotoga maritima ATCC 43589 Q9X0C6 AND Q9X0C8 Q9X0C6: subunit HisF, Q9X0C8: subunit HisH
-
Thermotoga maritima ATCC 43589 Q9X0C8 and Q9X0C6 Q9X0C8: subunit HisH, Q9X0C6: subunit HisF
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
 Thermotoga maritima 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine the enzyme is involved in histidine biosynthesis Thermotoga maritima 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
 Thermotoga maritima ATCC 43589 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine the enzyme is involved in histidine biosynthesis Thermotoga maritima ATCC 43589 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
 ?
L-glutamine + H2O
-
 Thermotoga maritima L-glutamate + NH3
-
 ?
L-glutamine + H2O binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70°C, compared to 4200-fold at 30°C Thermotoga maritima L-glutamate + NH3
-
 ?
L-glutamine + H2O binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70°C, compared to 4200-fold at 30°C Thermotoga maritima ATCC 43589 L-glutamate + NH3
-
 ?
L-glutamine + H2O
-
 Thermotoga maritima ATCC 43589 L-glutamate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
gps
-
 Thermotoga maritima
IGPS
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 binding of the allosteric effector ligand 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide stimulates millisecond timescale motions in IGPS that enhance its catalytic function. Allosteric activation decreases to 65-fold at 70°C, compared to 4200-fold at 30°C Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00372
-
 L-glutamine pH 8.0, 30°C Thermotoga maritima
0.00372
-
 L-glutamine pH 8.0, 30°C, basal activity Thermotoga maritima
0.0571
-
 L-glutamine pH 8.0, 40°C Thermotoga maritima
0.0571
-
 L-glutamine pH 8.0, 40°C, basal activity Thermotoga maritima
0.139
-
 L-glutamine pH 8.0, 50°C Thermotoga maritima
0.139
-
 L-glutamine pH 8.0, 50°C, basal activity Thermotoga maritima
0.204
-
 L-glutamine pH 8.0, 70°C Thermotoga maritima
0.204
-
 L-glutamine pH 8.0, 70°C, basal activity Thermotoga maritima
0.212
-
 L-glutamine pH 8.0, 60°C Thermotoga maritima
0.212
-
 L-glutamine pH 8.0, 60°C, basal activity Thermotoga maritima
4.09
-
 L-glutamine pH 8.0, 30°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
4.37
-
 L-glutamine pH 8.0, 40°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
4.89
-
 L-glutamine pH 8.0, 50°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
5.5
-
 L-glutamine pH 8.0, 60°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
5.92
-
 L-glutamine pH 8.0, 70°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Thermotoga maritima

General Information

General Information Comment Organism
metabolism the enzyme is involved in histidine biosynthesis Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000758
-
 L-glutamine pH 8.0, 30°C Thermotoga maritima
0.0121
-
 L-glutamine pH 8.0, 40°C Thermotoga maritima
0.0365
-
 L-glutamine pH 8.0, 50°C Thermotoga maritima
0.054
-
 L-glutamine pH 8.0, 60°C Thermotoga maritima
0.0689
-
 L-glutamine pH 8.0, 70°C Thermotoga maritima
0.121
-
 L-glutamine pH 8.0, 40°C, basal activity Thermotoga maritima
0.365
-
 L-glutamine pH 8.0, 50°C, basal activity Thermotoga maritima
0.544
-
 L-glutamine pH 8.0, 60°C, basal activity Thermotoga maritima
0.688
-
 L-glutamine pH 8.0, 70°C, basal activity Thermotoga maritima
0.758
-
 L-glutamine pH 8.0, 30°C, basal activity Thermotoga maritima
2980
-
 L-glutamine pH 8.0, 50°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
3150
-
 L-glutamine pH 8.0, 30°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
3240
-
 L-glutamine pH 8.0, 40°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
4040
-
 L-glutamine pH 8.0, 60°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima
4480
-
 L-glutamine pH 8.0, 70°C, activated by 5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide Thermotoga maritima