Literature summary for 4.3.2.10 extracted from

Holinski, A.; Heyn, K.; Merkl, R.; Sterner, R.
Combining ancestral sequence reconstruction with protein design to identify an interface hotspot in a key metabolic enzyme complex (2017), Proteins, 85, 312-321 .

Search for more literature for 4.3.2.10

Cloned(Commentary)

Cloned (Comment)	Organism
expression of HisF from Pyrobaculum arsenaticum {DSM 13514} in Escherichia coli BL21-Gold (DE3)	Zymomonas mobilis
expression of HisF from Thermotoga maritima or Pyrobaculum arsenaticum {DSM 13514} in Escherichia coli BL21-Gold (DE3)	Pyrobaculum arsenaticum

Organism

Organism	UniProt	Comment	Textmining
Pyrobaculum arsenaticum	A4WHB6 AND A4WHA5	A4WHB6: subunit HisF, A4WHA5: subunit HisH	-
Pyrobaculum arsenaticum DSM 13514	A4WHB6 AND A4WHA5	A4WHB6: subunit HisF, A4WHA5: subunit HisH	-
Zymomonas mobilis	Q5NMD6 AND Q5NMD4	Q5NMD6: subunit HisF, Q5NMD4: subunit HisH	-
Zymomonas mobilis ZM4	Q5NMD6 AND Q5NMD4	Q5NMD6: subunit HisF, Q5NMD4: subunit HisH	-

Purification (Commentary)

Purification (Comment)	Organism
-	Zymomonas mobilis
-	Pyrobaculum arsenaticum

Subunits

Subunits	Comment	Organism
More	subunit HisH from Zymomonas mobilis (zmHisH) binds with high affinity to the reconstructed subunit HisF from the last universal common ancestor (LUCA-HisF) but not to subunit HisF from Pyrobaculum arsenaticum (paHisF), which differ by 103 residues. Phe74 of LUCA-HisF is decisive for its affinity for zmHisH	Zymomonas mobilis
More	subunit HisH from Zymomonas mobilis (zmHisH) binds with high affinity to the reconstructed subunit HisF from the last universal common ancestor (LUCA-HisF) but not to subunit HisF from Pyrobaculum arsenaticum (paHisF), which differ by 103 residues. Phe74 of LUCA-HisF is decisive for its affinity for zmHisH	Pyrobaculum arsenaticum

Synonyms

Synonyms	Comment	Organism
HisF:HisH complex	-	Zymomonas mobilis
HisF:HisH complex	-	Pyrobaculum arsenaticum
ImGP synthase	-	Zymomonas mobilis
ImGP synthase	-	Pyrobaculum arsenaticum

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Release 2023.1 (January 2023)