BRENDA - Enzyme Database
show all sequences of 4.3.2.10

Structural evidence for ammonia tunneling across the (beta alpha)(8) barrel of the imidazole glycerol phosphate synthase bienzyme complex

Douangamath, A.; Walker, M.; Beismann-Driemeyer, S.; Vega-Fernandez, M.C.; Sterner, R.; Wilmanns, M.; Structure 10, 185-193 (2002)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
vapor diffusion method
Thermotoga maritima
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Thermotoga maritima
the enzyme links histidine and de novo purine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Thermotoga maritima ATCC 43589
the enzyme links histidine and de novo purine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Thermotoga maritima
Q9X0C6 AND Q9X0C8
Q9X0C6: subunit HisF, Q9X0C8: subunit HisH
-
Thermotoga maritima ATCC 43589
Q9X0C6 AND Q9X0C8
Q9X0C6: subunit HisF, Q9X0C8: subunit HisH
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thermotoga maritima
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
749381
Thermotoga maritima
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme links histidine and de novo purine biosynthesis
749381
Thermotoga maritima
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
749381
Thermotoga maritima ATCC 43589
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme links histidine and de novo purine biosynthesis
749381
Thermotoga maritima ATCC 43589
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
Subunits
Subunits
Commentary
Organism
heterodimer
the structure of the enzyme provides a model how ammonia is channeled over a distance of about 25 A. The larger part of the putative ammonia tunnel is provided by the interior of the beta barrel of subunit HisF, which has a (betaalpha)8 fold
Thermotoga maritima
Synonyms
Synonyms
Commentary
Organism
ImGP synthase
-
Thermotoga maritima
Crystallization (Commentary) (protein specific)
Crystallization
Organism
vapor diffusion method
Thermotoga maritima
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Thermotoga maritima
the enzyme links histidine and de novo purine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
Thermotoga maritima ATCC 43589
the enzyme links histidine and de novo purine biosynthesis
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermotoga maritima
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
749381
Thermotoga maritima
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme links histidine and de novo purine biosynthesis
749381
Thermotoga maritima
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
-
749381
Thermotoga maritima ATCC 43589
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
5-[(5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine
the enzyme links histidine and de novo purine biosynthesis
749381
Thermotoga maritima ATCC 43589
5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + L-glutamate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
heterodimer
the structure of the enzyme provides a model how ammonia is channeled over a distance of about 25 A. The larger part of the putative ammonia tunnel is provided by the interior of the beta barrel of subunit HisF, which has a (betaalpha)8 fold
Thermotoga maritima
General Information
General Information
Commentary
Organism
metabolism
the enzyme links histidine and de novo purine biosynthesis
Thermotoga maritima
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme links histidine and de novo purine biosynthesis
Thermotoga maritima
Other publictions for EC 4.3.2.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747861
Lisi
Glutamine hydrolysis by imida ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Front. Mol. Biosci.
5
4
2018
1
-
2
-
-
-
-
15
-
-
-
2
-
9
-
-
2
-
-
-
-
-
8
-
2
1
1
-
15
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
15
-
-
-
2
-
-
-
2
-
-
-
-
8
-
1
1
-
15
1
-
-
-
-
1
1
-
15
15
749186
Lisi
Altering the allosteric pathw ...
Thermotoga maritima
Proc. Natl. Acad. Sci. USA
114
E3414-E3423
2017
-
2
-
-
4
-
-
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
10
-
-
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
10
-
-
-
-
-
1
1
-
10
10
749258
Holinski
Combining ancestral sequence ...
Pyrobaculum arsenaticum, Zymomonas mobilis, Pyrobaculum arsenaticum DSM 13514, Zymomonas mobilis ZM4
Proteins
85
312-321
2017
-
-
2
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747112
Rivalta
Allosteric communication disr ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Biochemistry
55
6484-6494
2016
-
2
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
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2
-
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-
-
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1
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-
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-
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749239
Liebold
The interaction of ammonia an ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Protein Sci.
19
1774-1782
2010
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
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-
-
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1
-
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1
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1
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748246
Lipchock
Millisecond dynamics in the a ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
J. Biomol. NMR
45
73-84
2009
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
747285
Amaro
Structural elements in IGP sy ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Biophys. J.
89
475-487
2005
-
-
-
-
3
-
-
2
-
-
-
2
-
5
-
-
1
-
-
-
-
-
6
-
1
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
2
-
-
-
1
-
-
-
-
6
-
-
-
-
2
1
-
-
-
-
1
1
-
2
2
747057
Chaudhuri
Toward understanding the mech ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Biochemistry
42
7003-7012
2003
-
-
-
1
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
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6
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
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1
-
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2
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-
-
-
-
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6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
747058
Myers
Substrate-induced changes in ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Biochemistry
42
7013-7022
2003
-
-
-
-
7
-
3
16
-
-
-
-
-
5
-
-
-
-
-
-
-
-
4
-
1
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
3
-
16
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
16
-
-
-
-
-
-
-
-
16
16
748120
Omi
Structure of imidazole glycer ...
Thermus thermophilus
J. Biochem.
132
759-765
2002
-
-
1
1
-
-
-
-
-
-
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1
-
1
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1
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-
-
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2
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1
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1
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1
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1
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2
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-
-
-
-
-
-
-
1
1
-
-
-
749381
Douangamath
Structural evidence for ammon ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
Structure
10
185-193
2002
-
-
-
1
-
-
-
-
-
-
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2
-
6
-
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1
-
-
-
-
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4
1
1
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-
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1
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2
-
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1
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-
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4
1
-
-
-
-
-
-
-
-
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1
1
-
-
-
746612
Banfield
Structure of HisF, a histidin ...
Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523
Acta Crystallogr. Sect. D
57
1518-1525
2001
-
-
1
1
-
-
-
-
-
-
-
-
-
2
-
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1
-
-
-
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1
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1
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1
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-
-
-
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-
748148
Beismann-Driemeyer
Imidazole glycerol phosphate ...
Thermotoga maritima, Thermotoga maritima ATCC 43589
J. Biol. Chem.
276
20387-20396
2001
-
-
1
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7
-
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4
-
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2
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6
-
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1
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6
1
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4
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1
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7
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4
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2
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1
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6
1
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-
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4
-
-
-
-
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1
1
-
4
4
749228
Chittur
Expression and purification o ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Protein Expr. Purif.
18
366-377
2000
-
-
1
-
-
-
-
3
-
-
5
2
-
5
-
-
1
-
-
-
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1
6
2
2
-
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3
2
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-
-
-
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1
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-
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-
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3
-
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5
2
-
-
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1
-
-
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1
6
2
-
-
-
3
2
-
-
-
-
1
1
-
3
3
747754
Fujimori
An Arabidopsis cDNA encoding ...
Arabidopsis thaliana
FEBS Lett.
428
229-234
1998
-
-
-
-
-
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-
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1
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7
-
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2
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1
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747052
Klem
Imidazole glycerol phosphate ...
Escherichia coli, Escherichia coli K12
Biochemistry
32
5177-5186
1993
2
-
1
-
-
-
-
4
-
-
1
2
-
3
-
-
1
-
-
-
-
-
6
1
2
1
-
-
4
2
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
4
-
-
1
2
-
-
-
1
-
-
-
-
6
1
1
-
-
4
2
1
-
-
-
1
1
-
4
4