Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminoglutaryl-L-1-aminobutyrate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
5-L-Glutamyl-L-alpha-aminobutyrate | isoenzyme with pI 5.1, in extract, Km increases 4-10fold during purification | Rattus norvegicus | |
6.6 | - |
5-L-Glutamyl-L-alpha-aminobutyrate | isoenzyme with pI 4.6 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
1 * 27000, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoenzyme with pI 5.1: ammonium sulfate, heat, Sephadex G-75, DEAE-cellulose, thiol-Sepharose 4B, isoenzyme with pI 4.6: ammonium sulfate, heat, CM-cellulose, Sephadex G-75, DEAE-cellulose, ioselectric focusing, gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158 | - |
pI 4.6 isoenzyme | Rattus norvegicus |
181 | - |
pI 5.1 isoenzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, pI 4.6-isoform, at least 2 months | Rattus norvegicus |
0°C, pI 5.1-isoform, 3 weeks, 30% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate | model substrate, in vivo and in vitro | Mus musculus | 3-methyl-5-oxoproline + L-alpha-aminobutyrate | - |
? | |
5-L-(threo-4-methyl)glutamyl-L-2-aminobutyrate | model substrate, in vivo and in vitro | Rattus norvegicus | 3-methyl-5-oxoproline + L-alpha-aminobutyrate | - |
? | |
5-L-glutamyl-5-L-glutamyl-glycine | high activity | Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-glycine | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-alanine | high activity | Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-alanine | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-amino acid | - |
Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-amino acid | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-glutamate | - |
Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-glutamate | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-glutamine | high activity | Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-glutamine | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-leucine | - |
Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-leucine | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-lysine | - |
Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-lysine | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-phenylalanine | - |
Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-phenylalanine | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-proline | - |
Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-proline | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-threonine | high activity | Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-threonine | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-tyrosine | - |
Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-tyrosine | - |
? | |
5-L-glutamyl-5-L-glutamyl-L-valine | - |
Rattus norvegicus | 5-oxoproline + 5-L-glutamyl-L-valine | - |
? | |
5-L-glutamyl-cysteine | - |
Rattus norvegicus | 5-oxoproline + L-cysteine | - |
? | |
5-L-glutamyl-glycine | poor substrate | Rattus norvegicus | 5-oxoproline + glycine | - |
? | |
5-L-glutamyl-L-5-L-glutamyl-L-4-nitroanilide | - |
Rattus norvegicus | 5-L-oxoproline + 5-L-glutamyl-L-p-nitroanilide | - |
? | |
5-L-glutamyl-L-alanine | - |
Rattus norvegicus | 5-L-oxoproline + L-alanine | - |
? | |
5-L-glutamyl-L-alpha-aminobutyrate | - |
Mus musculus | 5-L-oxoproline + L-alpha-aminobutyrate | - |
? | |
5-L-glutamyl-L-alpha-aminobutyrate | - |
Rattus norvegicus | 5-L-oxoproline + L-alpha-aminobutyrate | - |
? | |
5-L-glutamyl-L-amino acid | - |
Mus musculus | 5-oxoproline + L-amino acid | i.e. 2-pyrrolidone-5-carboxylic acid | ? | |
5-L-glutamyl-L-amino acid | - |
Rattus norvegicus | 5-oxoproline + L-amino acid | i.e. 2-pyrrolidone-5-carboxylic acid | ? | |
5-L-glutamyl-L-aspartate | - |
Rattus norvegicus | 5-oxoproline + L-aspartate | - |
? | |
5-L-glutamyl-L-cysteine | - |
Rattus norvegicus | 5-oxoproline + L-cysteine | - |
? | |
5-L-glutamyl-L-glutamate | - |
Rattus norvegicus | 5-oxoproline + L-glutamate | - |
? | |
5-L-glutamyl-L-glutamine | best substrate | Rattus norvegicus | 5-oxoproline + L-glutamine | - |
? | |
5-L-glutamyl-L-isoleucine | very low activity | Rattus norvegicus | 5-oxoproline + L-isoleucine | - |
? | |
5-L-glutamyl-L-leucine | very low activity | Rattus norvegicus | 5-oxoproline + L-leucine | - |
? | |
5-L-glutamyl-L-methionine | - |
Rattus norvegicus | 5-oxoproline + L-methionine | - |
? | |
5-L-glutamyl-L-phenylalanine | poor substrate | Rattus norvegicus | 5-oxoproline + L-phenylalanine | - |
? | |
5-L-glutamyl-L-proline | very low activity | Rattus norvegicus | 5-oxoproline + L-proline | - |
? | |
5-L-glutamyl-L-tyrosine | very low activity | Rattus norvegicus | 5-oxoproline + L-tyrosine | - |
? | |
5-L-glutamyl-L-valine | very low activity | Rattus norvegicus | 5-oxoproline + L-valine | - |
? | |
5-L-glutamyl-L-valine | poor substrate | Rattus norvegicus | 5-oxoproline + L-valine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
two isozymic forms of different pI-values: pI 4.6 and 5.1 | Rattus norvegicus |
7.5 | 8 | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
2-aminoglutaryl-L-alpha-aminobutyrate | - |
Rattus norvegicus |