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Literature summary for 4.3.3.6 extracted from

  • Matsuura, A.; Yoon, J.Y.; Yoon, H.J.; Lee, H.H.; Suh, S.W.
    Crystal structure of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii (2012), Mol. Cells, 34, 407-412.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.3.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 (DE3) pLysS Pyrococcus horikoshii
overexpressed in Escherichia coli strain Rosetta2 (DE3) Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
2-methyl-2,4-pentanediol Pyrococcus horikoshii
crystallized at 23°C using 2-methyl-2,4-pentanediol as a precipitant. Crystals of apo and ribose 5'-phosphate complex forms of PdxS diffract to 2.7 A and 3.1 A resolution, respectively, and belong to the monoclinic space group P2(1) Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O59080
-
-
Pyrococcus horikoshii OT-3 O59080
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+
-
 Pyrococcus horikoshii pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?
D-ribose 5-phosphate + glyceraldehyde 3-phosphate + NH4+
-
 Pyrococcus horikoshii OT-3 pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer PdxS exists as a hexamer or dodecamer depending on species and makes a 1:1 complex with PdxT. Pyrococcus horikoshii PdxS forms hexamer in solution. It has a 37 amino acids insertion region, which is found in some archaeal PdxS proteins. This additional insertion perturbs dodecamer formation of Pyrococcus horikoshii PdxS Pyrococcus horikoshii
homohexamer gel filtration Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
PdxS
-
 Pyrococcus horikoshii
pyridoxal biosynthesis lyase PdxS
-
 Pyrococcus horikoshii