Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Codon Plus using expression vector pGEX-4T3/GST-CSE | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminooxyacetic acid | - |
Escherichia coli | |
Beta-cyanoalanine | - |
Escherichia coli | |
hydroxylamine | - |
Escherichia coli | |
isoniazid | - |
Escherichia coli | |
L-aminoethoxyvinylglycine | - |
Escherichia coli | |
additional information | no or poor inhibition by methylselenocysteine, D-cycloserine, pargyline, and parsalmide | Escherichia coli | |
propargylglycine | - |
Escherichia coli | |
trifluoroalanine | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | Escherichia coli | - |
L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
L-cysteine + H2O | Escherichia coli | - |
H2S + pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cystathionine + H2O | - |
Escherichia coli | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
L-cysteine + H2O | - |
Escherichia coli | H2S + pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CSE | - |
Escherichia coli |
cystathionine gamma lyase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.001 | - |
pH 8.2, 37°C | Escherichia coli | L-aminoethoxyvinylglycine | |
0.0011 | - |
pH 8.2, 37°C | Escherichia coli | aminooxyacetic acid | |
0.0048 | - |
pH 8.2, 37°C | Escherichia coli | hydroxylamine | |
0.014 | - |
pH 8.2, 37°C | Escherichia coli | Beta-cyanoalanine | |
0.04 | - |
pH 8.2, 37°C | Escherichia coli | propargylglycine | |
0.289 | - |
pH 8.2, 37°C | Escherichia coli | trifluoroalanine |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in H2S biosynthesis | Escherichia coli |