Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminoethoxyvinylglycine | irreversible | Arabidopsis thaliana | |
iodoacetamide | - |
Arabidopsis thaliana | |
L-alpha-(2-aminoethoxyvinyl)glycine | - |
Arabidopsis thaliana | |
NEM | - |
Arabidopsis thaliana | |
rhizobitoxine | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
4 * 46000, SDS-PAGE | Arabidopsis thaliana |
170000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystathionine + H2O | Arabidopsis thaliana | enzyme is involved in methionine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
var. columbia | - |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.5 | - |
- |
Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-80°C, stable for several months | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystathionine + H2O | enzyme is involved in methionine biosynthesis | Arabidopsis thaliana | ? | - |
? | |
L-cystathionine + H2O | - |
Arabidopsis thaliana | L-homocysteine + NH3 + pyruvate | - |
? | |
L-djenkolate + H2O | - |
Arabidopsis thaliana | pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 46000, SDS-PAGE | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: optimum, 90°C: 17% of maximal activity | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
15 min, 90% loss of activity | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | each subunit of the tetramer is associated with one molecule of pyridoxal 5'-phosphate. Schiff-base formation between the cofactor and the holoenzyme occurs at Lys278 | Arabidopsis thaliana |