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Literature summary for 4.4.1.13 extracted from

  • Gentry-Weeks, C.R.; Keith, J.M.; Thompson, J.
    Toxicity of Bordetella avium beta-cystathionase toward MC3T3-E1 osteogenic cells (1993), J. Biol. Chem., 268, 7298-7314.
    View publication on PubMed
Search for more literature for 4.4.1.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bordetella avium

General Stability

General Stability Organism
no decrease in activity after repeated freezing, -20°C, and thawing Bordetella avium

Inhibitors

Inhibitors Comment Organism Structure
DTT 10 mM, 19% inhibition Bordetella avium
hydroxylamine 1 mM, 92% inhibition Bordetella avium
iodoacetamide 10 mM, 61% inhibition Bordetella avium
iodoacetate 10 mM, 25% inhibition Bordetella avium
KCN 1 mM, 84% inhibition Bordetella avium
L-canaline 1 mM, 67% inhibition, 10 mM complete inhibition Bordetella avium
NEM 1 mM, 94% inhibition Bordetella avium
p-hydroxymercuribenzoate 1 mM, 92% inhibition Bordetella avium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 L-djenkolate
-
 Bordetella avium
0.07
-
 L-cystathionine
-
 Bordetella avium
0.104
-
 Lanthionine
-
 Bordetella avium
0.47
-
 S-(2-aminoethyl)-L-Cys
-
 Bordetella avium
0.67
-
 S-methyl-L-Cys
-
 Bordetella avium
5.3
-
 D-Cys
-
 Bordetella avium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 2 * 41000, SDS-PAGE Bordetella avium
42606
-
 2 * 42606, calculation from nucleotide sequence Bordetella avium
80000
-
 gel filtration Bordetella avium

Organism

Organism UniProt Comment Textmining
Bordetella avium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bordetella avium

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
-20°C, stable for 6 months Bordetella avium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Cys + H2O
-
 Bordetella avium ?
-
 ?
L-cystathionine + H2O
-
 Bordetella avium L-homocysteine + NH3 + pyruvate
-
 ?
L-cystine + H2O
-
 Bordetella avium L-thiocysteine + pyruvate + NH3
-
 ?
L-djenkolate + H2O
-
 Bordetella avium pyruvate
-
 ?
lanthionine + H2O
-
 Bordetella avium L-cysteine + NH3 + pyruvate
-
 ?
additional information osteotoxin is lethal for MC3T3-E1 osteogenic cells, fetal bovine trabecular cells, UMR106-01(BSP) rat osteosarcoma cells and embryonic bovine tracheal cells. The cytotoxicity can be attributed to: 1. beta-cystathionase-catalyzed cleavage of L-cystine in the medium and formation of reactive sulfane-containing derivatives, 2. Transfer of sulfane sulfur to metabolically sensitive or structurally important proteins in the osteogenic cells Bordetella avium ?
-
 ?
S-(2-aminoethyl)-L-Cys + H2O
-
 Bordetella avium ?
-
 ?
S-methyl-L-Cys + H2O
-
 Bordetella avium ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Bordetella avium
dimer 2 * 42606, calculation from nucleotide sequence Bordetella avium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 room temperature, t1/2: 24 min Bordetella avium
25 40 stable, rapid decline in activity above 40°C Bordetella avium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Bordetella avium

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate each subunit of the dimer contains one molecule of pyridoxal 5'-phosphate Bordetella avium