Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyruvate | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C364A | increased activity towards L-selenocysteine and L-cysteine sulfinate, activity toward L-cysteine was completely abolished | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
L-selenocysteine | in the presence of pyruvate, pH 7.5 | Escherichia coli | |
30 | - |
L-cysteine sulfinate | in the presence of pyruvate, pH 7.5 | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-selenocysteine + reduced acceptor | Escherichia coli | - |
selenide + L-alanine + acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
overexpressed protein | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine sulfinic acid + reduced acceptor | - |
Escherichia coli | SO2 + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | - |
Escherichia coli | selenide + L-alanine + acceptor | - |
? | |
L-selenocysteine + reduced acceptor | enzyme bound pyridoxal 5'-phosphate serves as acceptor | Escherichia coli | selenide + L-alanine + acceptor | - |
? | |
additional information | traces of activity with L-cysteine | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | two other proteins (CDS and IscS) with activity towards L-selenocysteine identified that are much less specific | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | required | Escherichia coli |