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Literature summary for 4.4.1.17 extracted from

  • San Francisco, B.; Bretsnyder, E.C.; Kranz, R.G.
    Human mitochondrial holocytochrome c synthases heme binding, maturation determinants, and complex formation with cytochrome c (2013), Proc. Natl. Acad. Sci. USA, 110, E788-E797.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.4.1.17

Cloned(Commentary)

Cloned (Comment) Organism
coexpression of N-terminally GST-tagged wild-type enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants in Escherichia coli Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane membrane-bound Homo sapiens 31966
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, recombinant GST-tagged enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Apocytochrome c + heme Homo sapiens
-
 Holocytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P53701
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type membrane-bound human GST-tagged enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants from Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Apocytochrome c + heme
-
 Homo sapiens Holocytochrome c
-
 ?
Apocytochrome c + heme conserved His154 is the key ligand to the heme iron, formation of the enzyme-heme complex serves as the platform for interaction with apocytochrome c, the heme is the central molecule mediating contact between enzyme and apocytochrome c. Conserved His19 of the CXXCH motif in apocytochrome c supplies the second axial ligand to heme in the trapped enzyme-heme-cytochrome c complex. Molecular mechanisms, overview Homo sapiens Holocytochrome c
-
 ?
additional information substrate specificity of the human enzyme, overview. Engineering of a bacterial cytochrome c into a robust substrate for the human enzyme, overview Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, recombinant GST-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
HCCS
-
 Homo sapiens
Holocytochrome c synthetase
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function cytochrome c, an essential electron carrier in mitochondria and a critical component of the apoptotic pathway, contains a heme cofactor covalently attached to the protein at a conserved CXXCH motif requiring the mitochondrial protein holocytochrome c synthase Homo sapiens