Cloned (Comment) | Organism |
---|---|
overexpression in fission yeast with a His6 tag at the C terminus | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
LTC4S crystallized with glutathione, The structure is determined by the multiwavelength anomalous diffraction method by using the diffraction images from native LTC4S and a selenomethionine-substituted Leu121Met mutant | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L121M | by site-directed mutagenesis to prepare the selenomethionine-substituted LTC4S, the mutant yeast clone is grown with selenomethionine, purified and crystallized similarly to the wild-type enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
- |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16873 | - |
- |
Purification (Comment) | Organism |
---|---|
solubilized with sodium deoxycholic acid and DDM, four steps of column chromatography, including affinity chromatography with S-hexyl GSH | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + glutathione | - |
Homo sapiens | leukotriene C4 | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
leukotriene C4 synthetase | - |
Homo sapiens |
LTC4 synthase | - |
Homo sapiens |
LTC4S | - |
Homo sapiens |