Cloned (Comment) | Organism |
---|---|
preparation of pRluc-LTC4S and pGFPLTC4S (full length and truncated forms). Full length and truncated forms of LTC4S excised from the pGFP vector and subcloned in frame with GST in a pGEX vector and transformed into Escherichia coli Y1090. Full length LTC4S subcloned into a pDsRed vector. HEK 293/T cells co-transfected with pRluc-LTC4S and pGFP fusion constructs. COS-7 cells co-transfected with GFP-5-lipoxygenase activating protein and dsRed-LTC4S | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | 5-lipoxygenase activating protein- and LTC4S-fusion proteins co-localize | Homo sapiens | 5783 | - |
nucleus | 5-lipoxygenase activating protein- and LTC4S-fusion proteins co-localize at the nuclear envelope | Homo sapiens | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q16873 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
leukotriene C4 synthase | - |
Homo sapiens |
LTC4S | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | LTC4S interacts in vitro with both 5-lipoxygenase activating protein and 5-lipoxygenase, these interactions involve distinct parts of LTC4S. 5-lipoxygenase activating protein binds to the N-terminal part/first hydrophobic region of LTC4S, whereas 5-lipoxygenase binds to the second hydrophilic loop of LTC4S. Direct interaction of 5-lipoxygenase and LTC4S in ionophore-stimulated (but not unstimulated) cells | Homo sapiens |