Literature summary for 4.4.1.20 extracted from

Rinaldo-Matthis, A.; Wetterholm, A.; Martinez Molina, D.; Holm, J.; Niegowski, D.; Ohlson, E.; Nordlund, P.; Morgenstern, R.; Haeggstroem, J.Z.
Arginine 104 is a key catalytic residue in leukotriene C4 synthase (2010), J. Biol. Chem., 285, 40771-40776.

Search for more literature for 4.4.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
the interaction of residue R104 with the thiol group of GSH reduces its pKa to allow formation of a thiolate anion and subsequent nucleophilic attack at C6 of LTA4.Crystal structure of mutant R31Q at 2.1 A reveals a Q31 side chain pointing away from the active site	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
R104A	5.7% of wild-type activity	Homo sapiens
R104K	0.1% of wild-type activity	Homo sapiens
R104S	4.6% of wild-type activity	Homo sapiens
R104T	1.1% of wild-type activity	Homo sapiens
R31A	12% of wild-type activity	Homo sapiens
R31Q	30% of wild-type activity	Homo sapiens
R51A	57% of wild-type activity	Homo sapiens
R51Q	29% of wild-type activity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	leukotriene A4	mutant R31A, 22°C, pH not specified in the publication	Homo sapiens
0.07	-	leukotriene A4	mutant R31Q, 22°C, pH not specified in the publication	Homo sapiens
0.1	-	leukotriene A4	mutant R104S, 22°C, pH not specified in the publication	Homo sapiens
0.3	-	leukotriene A4	wild-type, 22°C, pH not specified in the publication	Homo sapiens
0.5	-	leukotriene A4	mutant R104A, 22°C, pH not specified in the publication	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q16873	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.044	-	mutant R104K, 22°C, pH not specified in the publication	Homo sapiens
0.4	-	mutant R104T, 22°C, pH not specified in the publication	Homo sapiens
1.6	-	mutant R104S, 22°C, pH not specified in the publication	Homo sapiens
2	-	mutant R104A, 22°C, pH not specified in the publication	Homo sapiens
4.3	-	mutant R31A, 22°C, pH not specified in the publication	Homo sapiens
10	-	mutant R51Q, 22°C, pH not specified in the publication	Homo sapiens
10.5	-	mutant R31Q, 22°C, pH not specified in the publication	Homo sapiens
20	-	mutant R51A, 22°C, pH not specified in the publication	Homo sapiens
35	-	wild-type, 22°C, pH not specified in the publication	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
leukotriene A4 + glutathione	-	Homo sapiens	leukotriene C4	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.5	-	leukotriene A4	mutant R104S, 22°C, pH not specified in the publication	Homo sapiens
0.66	-	leukotriene A4	mutant R104A, 22°C, pH not specified in the publication	Homo sapiens
1.4	-	leukotriene A4	mutant R31A, 22°C, pH not specified in the publication	Homo sapiens
1.8	-	leukotriene A4	mutant R31Q, 22°C, pH not specified in the publication	Homo sapiens
11.7	-	leukotriene A4	wild-type, 22°C, pH not specified in the publication	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.3	-	leukotriene A4	mutant R104A, 22°C, pH not specified in the publication	Homo sapiens
5	-	leukotriene A4	mutant R104S, 22°C, pH not specified in the publication	Homo sapiens
26	-	leukotriene A4	mutant R31Q, 22°C, pH not specified in the publication	Homo sapiens
28	-	leukotriene A4	mutant R31A, 22°C, pH not specified in the publication	Homo sapiens
39	-	leukotriene A4	wild-type, 22°C, pH not specified in the publication	Homo sapiens

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Release 2023.1 (January 2023)