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Literature summary for 4.4.1.9 extracted from

  • Ikegami, F.; Takayama, K.; Tajima, Ch.; Murakoshi, I.
    Purification and properties of beta-cyano-L-alanine synthase from Spinacia oleracea (1988), Phytochemistry, 27, 2011-2016.
No PubMed abstract available
Search for more literature for 4.4.1.9

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 NaCN
-
 Spinacia oleracea
2.3
-
 L-cysteine
-
 Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, SDS-PAGE Spinacia oleracea
60000
-
 gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
212
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + HCN
-
 Spinacia oleracea H2S + L-3-cyanoalanine
-
 ir
L-cysteine + methanethiol
-
 Spinacia oleracea S-methyl-L-cysteine + ?
-
 ?
L-cysteine + NaCN
-
 Spinacia oleracea L-3-cyanoalanin + Na2S
-
 ?
L-cysteine + pyrazole
-
 Spinacia oleracea beta-(pyrazol-1-yl)-L-alanine + ?
-
 ?
additional information
-
 Spinacia oleracea ?
-
 ?
O-acetylserine + H2S
-
 Spinacia oleracea cysteine + acetic acid
-
 ir
O-acetylserine + HCN
-
 Spinacia oleracea beta-cyanoalanine + acetic acid
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 9.5
-
 Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 1 mol per mol of subunit Spinacia oleracea