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Literature summary for 4.6.1.15 extracted from

  • Cabezas, A.; Pinto, R.M.; Fraiz, F.; Canales, J.; Gonzalez-Santiago, S.; Cameselle, J.C.
    Purification, characterization, and substrate and inhibitor structure-activity studies of rat liver FAD-AMP lyase (cyclizing): Preference for FAD and specificity for splitting ribonucleoside diphosphate-X into ribonucleotide and a five-atom cyclic phosphodiester of X, either a monocyclic compound or a cis-bicyclic phosphodiester-pyranose fusion (2001), Biochemistry, 40, 13710-13722.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
Co2+ activates Rattus norvegicus
Mn2+ activates Rattus norvegicus
additional information thirty-five compounds structurally related to FAD tested Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Rattus norvegicus
ATP
-
 Rattus norvegicus
FAD concentration higher than 0.3 mM Rattus norvegicus
additional information twelve nucleotidic compounds tested, not inhibited by FMN, cFMN, dADP, dATP, AMP Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 FAD Mn2+, pH: 7.5 Rattus norvegicus
0.036 0.045 FAD Mn2+, pH: 8.3 Rattus norvegicus
0.09
-
 FAD Co2+, pH: 7.5 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme-activating cation Rattus norvegicus
Mn2+ enzyme-activating cation Rattus norvegicus
additional information Mg2+ , Ca2+, Zn2+, Ni2+, Cu2+, Fe3+, Li+, Na+, K+ not required for activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 SDS-PAGE Rattus norvegicus
100000
-
 ultrafiltration Rattus norvegicus
140000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FAD Rattus norvegicus
-
 AMP + riboflavin cyclic-4',5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
 female Wistar rats
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on DEAE-cellulose Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-80°C, TE: buffer 20 mM Tris-HCl and 0.5 mM EDTA, pH 8.2 at 4°C, 10 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FAD
-
 Rattus norvegicus AMP + riboflavin cyclic-4',5'-phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.82
-
 FAD Mn2+, pH: 7.5 Rattus norvegicus
19.3 24.1 FAD Mn2+, pH: 8.3 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 Co2+ as activating cation Rattus norvegicus
8.5
-
 Mn2+ as activating cation Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000025
-
 ADP
-
 Rattus norvegicus
0.00005
-
 ATP
-
 Rattus norvegicus