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Literature summary for 4.6.1.2 extracted from

  • Mayer, B.; Kleschyov, A.L.; Stessel, H.; Russwurm, M.; Munzel, T.; Koesling, D.; Schmidt, K.
    Inactivation of soluble guanylate cyclase by stoichiometric S-nitrosation (2008), Mol. Pharmacol., 75, 886-891.
    View publication on PubMed
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
2,2-diethyl-1-nitroso-oxyhydrazine 70-80% activation at 0.01 mM in presence of 2 mM GSH Bos taurus
NO binding of NO to a regulatory heme group at the beta-subunit of the protein results in 100fold stimulation of cGMP formation, NO is involved in the vascular NO/cGMP signalling, dysfunction of the signaling is thought to contribute to a wide variety of cardiovascular disorders Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
DNIC-G a dinitrosyl iron complex, solutions contain 18fold molar excess of free thiosulfate, inhibition of the NO-stimulated enzyme, inhibited by GSH Bos taurus
DNIC-Y a dinitrosyl iron complex, no free thiosulfate in solutions of the binuclear complex DNIC-Y, inhibition of the NO-stimulated enzyme, inhibited by GSH Bos taurus
additional information desensitization of the enzyme to NO and inactivation of soluble guanylate cyclase by stoichiometric S-nitrosation through dinitrosyl iron complexes, which is slowly reversible by GSH, overview Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Bos taurus
-
 3',5'-cyclic GMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from lung Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Bos taurus 3',5'-cyclic GMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Bos taurus

Synonyms

Synonyms Comment Organism
sGC
-
 Bos taurus
soluble guanylate cyclase
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Bos taurus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
 pH 7.4, 37°C, inhibition of NO-stimulated enzyme Bos taurus DNIC-G
0.01
-
 pH 7.4, 37°C, inhibition of NO-stimulated enzyme Bos taurus DNIC-Y