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Literature summary for 4.6.1.24 extracted from

  • Kumar, K.; Walz, F.G., Jr.
    Probing functional perfection in substructures of ribonuclease T1: double combinatorial random mutagenesis involving Asn43, Asn44, and Glu46 in the guanine binding loop (2001), Biochemistry, 40, 3748-3757.
    View publication on PubMed
Search for more literature for 4.6.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
N43G as active as the wild type enzyme Aspergillus oryzae
N43H as active as the wild type enzyme Aspergillus oryzae
N43I as active as the wild type enzyme Aspergillus oryzae
N43Q/N44G less active than the wild type enzyme Aspergillus oryzae
N43T as active as the wild type enzyme Aspergillus oryzae
N43Y as active as the wild type enzyme Aspergillus oryzae
N44H less active than the wild type enzyme Aspergillus oryzae
Q46_G47insL mutant with a Leu insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Q46_N47insQ mutant with a Glu insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Q46_P47insP mutant with a Pro insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Q46_P47insQ mutant with a Glu insertion in 47, as active as the wild type enzyme Aspergillus oryzae
Q46_P47insY mutant with a Tyr insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Q46_Q47insP mutant with a Pro insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Q46_R47insR mutant with an Arg insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Q46_S47insG mutant with a Gly insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Q46_S47insS mutant with a Ser insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Q46_T47insS mutant with a Ser insertion in 47, less active than the wild type enzyme Aspergillus oryzae
Y45S as active as the wild type enzyme Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43H mutant Aspergillus oryzae
0.0581
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43G mutant Aspergillus oryzae
0.0757
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, Q46_P47insQ mutant Aspergillus oryzae
0.0758
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43I mutant Aspergillus oryzae
0.0772
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43T mutant Aspergillus oryzae
0.081
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43Q/N44G mutant Aspergillus oryzae
0.092
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43T mutant Aspergillus oryzae
0.135
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.156
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N44H mutant Aspergillus oryzae
0.17
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, Y45S mutant Aspergillus oryzae
0.173
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43H mutant Aspergillus oryzae
0.19
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N44H mutant Aspergillus oryzae
0.259
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43Y mutant Aspergillus oryzae
0.321
-
 RNA pH 7.5, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.361
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, Q46_Q47insP mutant Aspergillus oryzae
0.433
-
 RNA pH 7.5, 25ÂșC, Y45S Aspergillus oryzae
0.44
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, wild-type enzyme Aspergillus oryzae
0.506
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, Q46_P47insP mutant Aspergillus oryzae
0.549
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43Q/N44G mutant Aspergillus oryzae
0.606
-
 guanylyl-3',5'-cytidine pH 6.0, 25ÂșC, Y45S mutant Aspergillus oryzae
0.61
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43I mutant Aspergillus oryzae
0.698
-
 RNA pH 7.5, 25ÂșC, N43H Aspergillus oryzae
0.73
-
 RNA pH 7.5, 25ÂșC, N43G Aspergillus oryzae
0.961
-
 RNA pH 7.5, 25ÂșC, N43Y Aspergillus oryzae
1.55
-
 RNA pH 7.5, 25ÂșC, N43I Aspergillus oryzae
2.64
-
 RNA pH 7.5, 25ÂșC, N43T Aspergillus oryzae
4
-
 adenylyl-3',5'-cytidine pH 6.0, 25ÂșC, N43G mutant Aspergillus oryzae
4.56
-
 RNA pH 7.5, 25ÂșC, N43Q/N44G Aspergillus oryzae
6.94
-
 RNA pH 7.5, 25ÂșC, Q46PQ mutant Aspergillus oryzae
19.6
-
 RNA pH 7.5, 25ÂșC, N44H Aspergillus oryzae
1470
-
 RNA pH 7.5, 25ÂșC, Q46PP mutant Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange chromatography and gel filtration Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenylyl-3',5'-cytidine ApC Aspergillus oryzae adenosine-2',3'-cyclic phosphate + cytidine
-
 ?
ApC + H2O
-
 Aspergillus oryzae adenosine 3'-phosphate + cytidine
-
 ?
GpC + H2O
-
 Aspergillus oryzae guanosine 3'-phosphate + cytidine
-
 ?
guanylyl-3',5'-cytidine + H2O GpC Aspergillus oryzae guanosine 3'-phosphate + cytidine
-
 ?
RNA + H2O
-
 Aspergillus oryzae Hydrolyzed RNA
-
 ?