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Literature summary for 4.6.1.24 extracted from

  • Makarov, A.A.; Yakovlev, G.I.; Mitkevich, V.A.; Higgin, J.J.; Raines, R.T.
    Zinc(II)-mediated inhibition of ribonuclease Sa by an N-hydroxyurea nucleotide and its basis (2004), Biochem. Biophys. Res. Commun., 319, 152-156.
    View publication on PubMed
Search for more literature for 4.6.1.24

Inhibitors

Inhibitors Comment Organism Structure
3'-N-hydroxyurea-3'-deoxythymidine-5'-phosphate competitive, inhibition is enhanced by nearly 10fold in presence of Zn2 in wild-type enzyme, no enhancement in mutant enzyme E54Q Kitasatospora aureofaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.151
-
 poly(I) 25°C, pH 6.2, wild-type enzyme Kitasatospora aureofaciens
0.32
-
 poly(I) 25°C, pH 6.2, mutant enzyme E54Q Kitasatospora aureofaciens

Organism

Organism UniProt Comment Textmining
Kitasatospora aureofaciens P05798
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(I) + H2O
-
 Kitasatospora aureofaciens ?
-
 ?

Synonyms

Synonyms Comment Organism
Ribonuclease SA
-
 Kitasatospora aureofaciens
RNase Sa
-
 Kitasatospora aureofaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 poly(I) 25°C, pH 6.2, mutant enzyme E54Q Kitasatospora aureofaciens
189
-
 poly(I) 25°C, pH 6.2, wild-type enzyme Kitasatospora aureofaciens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.95
-
 3'-N-hydroxyurea-3'-deoxythymidine-5'-phosphate 25°C, pH 6.2, wild-type enzyme Kitasatospora aureofaciens
1.15
-
 3'-N-hydroxyurea-3'-deoxythymidine-5'-phosphate 25°C, pH 6.2, mutant enzyme E54Q Kitasatospora aureofaciens