Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.24 extracted from

  • Czaja, R.; Perbandt, M.; Betzel, C.; Hahn, U.
    Purine activity of RNase T1RV is further improved by substitution of Trp59 by tyrosine (2005), Biochem. Biophys. Res. Commun., 336, 882-889.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of RNase T1-T2 (K41E/Y42F/N43R/Y45W/E46N/W59Y), hanging drop vapor diffusion method, 2.1 A resolution Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
K41E/Y42F/N43R/Y45W/E46N/W59Y RNase T1-R2 contains the additional mutation W59Y compared to RNase T1-RV variant. RNase T1-RV is a variant where the specificity is changed from guanine to purine, accompanied with a reduced activity. The additional mutation W59Y increases the activity in comparison to variant RV to 425% Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyne K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae P00651
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenylyl-3',5'-cytidine + H2O kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) Aspergillus oryzae adenosine 3'-phosphate + cytidine
-
?
guanylyl-3',5'-cytidine + H2O kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) Aspergillus oryzae ?
-
?

Synonyms

Synonyms Comment Organism
RNase T1-R2 mutant RNase T1 K41E/Y42F/N43R/Y45W/E46N/W59Y Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyne K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) Aspergillus oryzae