Literature summary for 4.6.1.24 extracted from

Chao, W.C.; Shen, J.Y.; Lu, J.F.; Wang, J.S.; Yang, H.C.; Wee, K.; Lin, L.J.; Kuo, Y.C.; Yang, C.H.; Weng, S.H.; Huang, H.C.; Chen, Y.H.; Chou, P.T.
Probing water environment of Trp59 in ribonuclease T1: insight of the structure-water network relationship (2014), J. Phys. Chem. B, 119, 2157-2167.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of the enzyme in Trp-auxotrophic Escherichia coli, resulting in site-specifically replacement of Trp residues with (2,7-aza)Trp	Aspergillus oryzae

Crystallization (Commentary)

Crystallization (Comment)	Organism
RNase T1 crystal structure analysis, PDB ID 9RNT	Aspergillus oryzae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Aspergillus oryzae	-	-

Organism

Organism	UniProt	Comment	Textmining
Aspergillus oryzae	-	-	-

Renatured (Commentary)

Renatured (Comment)	Organism
the enzyme can be reversibly unfolded and refolded by heating and high concentration of denaturants, such as urea and guanidine hydrochloride, without forming noticeable amounts of aggregates	Aspergillus oryzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme cleaves single-stranded RNA by catalyzing the hydrolysis of phosphodiester bonds specifically at the 3'-side of guanosine nucleotides	Aspergillus oryzae	?	-	?

Subunits

Subunits	Comment	Organism
More	the secondary structure of RNase T1 includes a long alpha-helix and two beta-sheets connected by extended loop regions, structure of hydrated RNase T1 in loop-close and loop-open forms, overview	Aspergillus oryzae

Synonyms

Synonyms	Comment	Organism
ribonuclease T1	-	Aspergillus oryzae
RNase T1	-	Aspergillus oryzae

General Information

General Information	Comment	Organism
additional information	Trp59 may play an important role in folding as well as in modulating the geometry of the RNase T1 active site. Trp59-water pairs appear to preferentially participate in a hydrogen bond network incorporating polar amino acid moieties on the protein surface and bulk waters, providing the structural dynamic features of the connecting loop region in RNase T1, molecular dynamic simulations, overview	Aspergillus oryzae

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Release 2023.1 (January 2023)