Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Na2S2O4 | requirement of a reducing agent for activity | Pseudomonas chlororaphis |
Cloned (Comment) | Organism |
---|---|
oxdA gene, overexpression in Escherichia coli BL21-Codon-Plus(DE3)-RIL, sequencing, gene cluster organisation | Pseudomonas chlororaphis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-2-nitrobenzoate | weak inhibition | Pseudomonas chlororaphis | |
AgNO3 | very sensitive to, concentration-dependent, 1 mM, 85.8% inhibition | Pseudomonas chlororaphis | |
CO | 49% inhibition | Pseudomonas chlororaphis | |
diethyldithiocarbamate | 1 mM, 40.6% inhibition | Pseudomonas chlororaphis | |
hydroxylamine | concentration-dependent, 1 mM, 100% inhibition | Pseudomonas chlororaphis | |
iodoacetate | weak inhibition | Pseudomonas chlororaphis | |
KCN | 1 mM, 41.3% inhibition | Pseudomonas chlororaphis | |
additional information | not inhibited by chelating agents, such as 2,2-dipyridyl, o-phenanthroline, 8-hydroxyquinoline, EDTA, and by phenylmethanesulfonyl fluoride, diisopropyl fluorophosphate | Pseudomonas chlororaphis | |
N-ethylmaleimide | weak inhibition | Pseudomonas chlororaphis | |
p-chloromercuribenzoate | weak inhibition | Pseudomonas chlororaphis | |
phenylhydrazine | 1 mM, 71.1% inhibition | Pseudomonas chlororaphis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
(E/Z)-n-butyraldoxime | pH 7, 30°C | Pseudomonas chlororaphis | |
3.4 | - |
(E/Z)-pyridine-2-aldoxime | pH 7, 30°C | Pseudomonas chlororaphis | |
11 | - |
(E/Z)-acetaldoxime | pH 7, 30°C | Pseudomonas chlororaphis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | contains 1.58 mol Ca2+ per mol of homodimeric OxdA, may act as another cofactor | Pseudomonas chlororaphis | |
Fe2+ | contains 1.62 mol iron per mol of homodimeric OxdA, present in the heme molecule, the heme iron of the active enzyme is in the ferrous state | Pseudomonas chlororaphis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 40127, calculated from the amino acid sequence, 2 * 38000, SDS-PAGE | Pseudomonas chlororaphis |
40127 | - |
2 * 40127, calculated from the amino acid sequence, 2 * 38000, SDS-PAGE | Pseudomonas chlororaphis |
76200 | - |
OxdA reduced by Na2S2O4, gel filtration | Pseudomonas chlororaphis |
76400 | - |
OxdA without reducing conditions, gel filtration | Pseudomonas chlororaphis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas chlororaphis | involved in carbon-nitrogen triple bond synthesis, responsible for the metabolism of aldoxime in vivo | ? | - |
? | |
additional information | Pseudomonas chlororaphis B23 | involved in carbon-nitrogen triple bond synthesis, responsible for the metabolism of aldoxime in vivo | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas chlororaphis | - |
B23 | - |
Pseudomonas chlororaphis | Q7WSJ4 | - |
- |
Pseudomonas chlororaphis B23 | - |
B23 | - |
Pseudomonas chlororaphis B23 | Q7WSJ4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant OxdA | Pseudomonas chlororaphis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.758 | - |
pH 7, 30°C, butyraldoxime as substrate, under aerobic conditions | Pseudomonas chlororaphis |
197 | - |
pH 7, 30°C, butyraldoxime as substrate, under anaerobic reduced conditions, OxdA reduced by Na2S2O4 | Pseudomonas chlororaphis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E/Z)-acetaldoxime | - |
Pseudomonas chlororaphis | acetonitrile + H2O | - |
? | |
(E/Z)-acetaldoxime | - |
Pseudomonas chlororaphis B23 | acetonitrile + H2O | - |
? | |
(E/Z)-n-butyraldoxime | best substrate, accepts both the E- and the Z-forms of butyraldoxime as substrates | Pseudomonas chlororaphis | n-butyronitrile + H2O | butyronitrile does not act as substrate | ? | |
(E/Z)-n-butyraldoxime | best substrate, accepts both the E- and the Z-forms of butyraldoxime as substrates | Pseudomonas chlororaphis B23 | n-butyronitrile + H2O | butyronitrile does not act as substrate | ? | |
(E/Z)-pyridine-2-aldoxime | poor substrate | Pseudomonas chlororaphis | pyridine-2-nitrile + H2O | - |
? | |
aliphatic aldoxime | strong preference of aliphatic aldoximes over aromatic aldoximes | Pseudomonas chlororaphis | aliphatic nitrile + H2O | - |
? | |
aliphatic aldoxime | strong preference of aliphatic aldoximes over aromatic aldoximes | Pseudomonas chlororaphis B23 | aliphatic nitrile + H2O | - |
? | |
an aliphatic aldoxime | - |
Pseudomonas chlororaphis | an aliphatic nitrile + H2O | - |
? | |
an aliphatic aldoxime | - |
Pseudomonas chlororaphis B23 | an aliphatic nitrile + H2O | - |
? | |
additional information | not: butyronitrile, aromatic aldoximes, such as benzaldoxime, isonitrosoacetophenone, and pyridine-4-aldoxime | Pseudomonas chlororaphis | ? | - |
? | |
additional information | involved in carbon-nitrogen triple bond synthesis, responsible for the metabolism of aldoxime in vivo | Pseudomonas chlororaphis | ? | - |
? | |
additional information | not: butyronitrile, aromatic aldoximes, such as benzaldoxime, isonitrosoacetophenone, and pyridine-4-aldoxime | Pseudomonas chlororaphis B23 | ? | - |
? | |
additional information | involved in carbon-nitrogen triple bond synthesis, responsible for the metabolism of aldoxime in vivo | Pseudomonas chlororaphis B23 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40127, calculated from the amino acid sequence, 2 * 38000, SDS-PAGE | Pseudomonas chlororaphis |
Synonyms | Comment | Organism |
---|---|---|
aldoxime dehydratase | - |
Pseudomonas chlororaphis |
OxdA | - |
Pseudomonas chlororaphis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas chlororaphis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
after 15 min preincubation OxdA is reduced by Na2S2O4, under anaerobic conditions, 100% of enzyme activity | Pseudomonas chlororaphis |
25 | - |
after 15 min preincubation OxdA is reduced by Na2S2O4, under anaerobic conditions, 94% of enzyme activity | Pseudomonas chlororaphis |
30 | - |
after 15 min preincubation OxdA is reduced by Na2S2O4, under anaerobic conditions, 91% of enzyme activity | Pseudomonas chlororaphis |
35 | - |
after 15 min preincubation OxdA is reduced by Na2S2O4, under anaerobic conditions, 78% of enzyme activity | Pseudomonas chlororaphis |
40 | - |
after 15 min preincubation OxdA is reduced by Na2S2O4, under anaerobic conditions, 51% of enzyme activity | Pseudomonas chlororaphis |
45 | - |
after 15 min preincubation OxdA is reduced by Na2S2O4, under anaerobic conditions, 14% of enzyme activity | Pseudomonas chlororaphis |
50 | - |
after 15 min preincubation OxdA is reduced by Na2S2O4, under anaerobic conditions, 3.2% of enzyme activity | Pseudomonas chlororaphis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
(E/Z)-pyridine-2-aldoxime | pH 7, 30°C | Pseudomonas chlororaphis | |
324 | - |
(E/Z)-n-butyraldoxime | pH 7, 30°C | Pseudomonas chlororaphis | |
336 | - |
(E/Z)-acetaldoxime | pH 7, 30°C | Pseudomonas chlororaphis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
maximum at pH 5.5, another high activity level occurs at pH 9.4 | Pseudomonas chlororaphis |
5.5 | - |
- |
Pseudomonas chlororaphis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | active over a broad pH-range | Pseudomonas chlororaphis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement of FMN | Pseudomonas chlororaphis | |
protoheme IX | hemoprotein, the heme iron of the active enzyme is in the ferrous state, carries protoheme IX as the prosthetic group, 0.69 mol heme per mol of subunit | Pseudomonas chlororaphis |