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Literature summary for 4.99.1.4 extracted from

  • Raux-Deery, E.; Leech, H.K.; Nakrieko, K.A.; McLean, K.J.; Munro, A.W.; Heathcote, P.; Rigby, S.E.; Smith, A.G.; Warren, M.J.
    Identification and characterization of the terminal enzyme of siroheme biosynthesis from Arabidopsis thaliana: a plastid-located sirohydrochlorin ferrochelatase containing a 2FE-2S center (2005), J. Biol. Chem., 280, 4713-4721.
    View publication on PubMed
Search for more literature for 4.99.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
Co2+ inactivates the enzyme at high concentrations Arabidopsis thaliana
Fe2+ inactivates the enzyme at high concentrations Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information it is not possible to measure an accurate KM value for the metals as at low concentration of metal the assay is not sensitive enough, whereas at higher metal ion concentrations the enzyme is inactivated Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ inactivates the enzyme at high concentrations Arabidopsis thaliana
Fe2+ inactivates the enzyme at high concentrations Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sirohydrochlorin + Fe2+ Arabidopsis thaliana
-
 siroheme + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0054
-
 isolated recombinant enzyme, with ferrous iron as substrate Arabidopsis thaliana
0.0485
-
 isolated recombinant enzyme, with cobalt as substrate Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sirohydrochlorin + Co2+ pH 8.0 Arabidopsis thaliana cobalt-sirohydrochlorin + H+
-
 ?
sirohydrochlorin + Fe2+
-
 Arabidopsis thaliana siroheme + H+
-
 ?
sirohydrochlorin + Fe2+ pH 8.0 Arabidopsis thaliana siroheme + H+
-
 ?

Synonyms

Synonyms Comment Organism
sirohydrochlorin ferrochelatase
-
 Arabidopsis thaliana