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Literature summary for 4.99.1.4 extracted from

  • Saha, K.; Webb, M.E.; Rigby, S.E.; Leech, H.K.; Warren, M.J.; Smith, A.G.
    Characterization of the evolutionarily conserved iron-sulfur cluster of sirohydrochlorin ferrochelatase from Arabidopsis thaliana (2012), Biochem. J., 444, 227-237.
    View publication on PubMed
Search for more literature for 4.99.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C135A the mutant shows increased specific activity compared to the wild type enzyme Arabidopsis thaliana
C199A the mutant shows reduced specific activity compared to the wild type enzyme Arabidopsis thaliana
C210A the mutant shows increased specific activity compared to the wild type enzyme Arabidopsis thaliana
C213A the mutant shows reduced specific activity compared to the wild type enzyme Arabidopsis thaliana
C219A the mutant shows increased specific activity compared to the wild type enzyme Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains 1.93 mol Fe2+ per mol of protein Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18600
-
 2 * 18600, estimated from SDS-PAGE Arabidopsis thaliana
36010
-
 calculated from gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sirohydrochlorin + Fe2+ Arabidopsis thaliana
-
 siroheme + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.25
-
 mutant enzyme C199A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
36.08
-
 mutant enzyme C213A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
38.5
-
 wild type enzyme, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
43
-
 mutant enzyme C219A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
44.67
-
 mutant enzyme C135A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana
55.17
-
 mutant enzyme C210A, in 20 mM Tris/HCl (pH 8.0) and 10 mM NaCl, at 23°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sirohydrochlorin + Fe2+
-
 Arabidopsis thaliana siroheme + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 18600, estimated from SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
SirB
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
4Fe-4S-center
-
 Arabidopsis thaliana