Literature summary for 4.99.1.4 extracted from

Bali, S.; Rollauer, S.; Roversi, P.; Raux-Deery, E.; Lea, S.M.; Warren, M.J.; Ferguson, S.J.
Identification and characterization of the missing terminal enzyme for siroheme biosynthesis in alpha-proteobacteria (2014), Mol. Microbiol., 92, 153-163.

Search for more literature for 4.99.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 codonplus (RIPL) cells	Paracoccus pantotrophus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using in 0.2 M imidazole malate pH 5.5, 24% (w/v) PEG 600 at a ratio of 50:50 ratio of protein : mother liquor and 10% ethylene glycol	Paracoccus pantotrophus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
20000	-	x * 20000, SDS-PAGE	Paracoccus pantotrophus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sirohydrochlorin + Fe2+	Paracoccus pantotrophus	-	siroheme + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Paracoccus pantotrophus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
chelating Sepharose column chromatography	Paracoccus pantotrophus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sirohydrochlorin + Fe2+	-	Paracoccus pantotrophus	siroheme + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 20000, SDS-PAGE	Paracoccus pantotrophus

Synonyms

Synonyms	Comment	Organism
CbiX	-	Paracoccus pantotrophus
CysG	-	Paracoccus pantotrophus
Met8p	-	Paracoccus pantotrophus
SirB	-	Paracoccus pantotrophus
sirohydrochlorin-ferrochelatase	-	Paracoccus pantotrophus

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Release 2023.1 (January 2023)