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Literature summary for 5.1.1.10 extracted from

  • Kawakami, R.; Ohmori, T.; Sakuraba, H.; Ohshima, T.
    Identification of a novel amino acid racemase from a hyperthermophilic archaeon Pyrococcus horikoshii OT-3 induced by D-amino acids (2015), Amino Acids, 47, 1579-1587 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine 1 mM, completely blocks activity Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52392
-
calculated from sequence Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Leu Pyrococcus horikoshii the enzyme is likely responsible for utilization of d-amino acids for growth L-Leu
-
?
D-Met Pyrococcus horikoshii the enzyme is likely responsible for utilization of d-amino acids for growth L-Met
-
?
D-Phe Pyrococcus horikoshii the enzyme is likely responsible for utilization of d-amino acids for growth L-Phe
-
?
D-Phe Pyrococcus horikoshii OT-3 the enzyme is likely responsible for utilization of d-amino acids for growth L-Phe
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O57878
-
-
Pyrococcus horikoshii O57878 PH0138 gene encodes an amino acid racemase, AAR
-
Pyrococcus horikoshii OT-3 O57878
-
-
Pyrococcus horikoshii OT-3 O57878 PH0138 gene encodes an amino acid racemase, AAR
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
substrate: D-Ala, pH 8.0, 80°C Pyrococcus horikoshii
3.9
-
substrate: D-Val, pH 8.0, 80°C Pyrococcus horikoshii
10.5
-
substrate: D-Met, pH 8.0, 80°C Pyrococcus horikoshii
15.6
-
substrate: D-Leu, pH 8.0, 80°C Pyrococcus horikoshii
22.7
-
substrate: D-Phe, pH 8.0, 80°C Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine
-
Pyrococcus horikoshii L-alanine
-
r
D-isoleucine
-
Pyrococcus horikoshii L-isoleucine
-
r
D-Leu broad substrate specificity Pyrococcus horikoshii L-Leu
-
?
D-Leu the enzyme is likely responsible for utilization of d-amino acids for growth Pyrococcus horikoshii L-Leu
-
?
D-leucine
-
Pyrococcus horikoshii L-leucine
-
r
D-Met broad substrate specificity Pyrococcus horikoshii L-Met
-
?
D-Met the enzyme is likely responsible for utilization of d-amino acids for growth Pyrococcus horikoshii L-Met
-
?
D-methionine
-
Pyrococcus horikoshii L-methionine
-
r
D-Phe the enzyme is likely responsible for utilization of d-amino acids for growth Pyrococcus horikoshii L-Phe
-
?
D-Phe Phe is the most preferred substrate among the amino acids, broad substrate specificity Pyrococcus horikoshii L-Phe
-
?
D-Phe the enzyme is likely responsible for utilization of d-amino acids for growth Pyrococcus horikoshii OT-3 L-Phe
-
?
D-Phe Phe is the most preferred substrate among the amino acids, broad substrate specificity Pyrococcus horikoshii OT-3 L-Phe
-
?
D-phenylalanine
-
Pyrococcus horikoshii L-phenylalanine
-
r
D-serine
-
Pyrococcus horikoshii L-serine
-
r
D-tryptophan
-
Pyrococcus horikoshii L-tryptophan
-
r
D-tyrosine
-
Pyrococcus horikoshii L-tyrosine
-
r
D-valine
-
Pyrococcus horikoshii L-valine
-
r
L-alanine
-
Pyrococcus horikoshii D-alanine
-
r
L-leucine
-
Pyrococcus horikoshii D-leucine
-
r
L-Methionine
-
Pyrococcus horikoshii D-Methionine
-
r
L-Methionine
-
Pyrococcus horikoshii OT-3 D-Methionine
-
r
L-phenylalanine
-
Pyrococcus horikoshii D-phenylalanine
-
r
L-valine
-
Pyrococcus horikoshii D-valine
-
r
additional information no activity with Pro, Asp or Glu Pyrococcus horikoshii ?
-
?
additional information PH0138 gene encodes an amino acid racemase, AAR. No activity with L-Pro, D-Asp, and D-Glu Pyrococcus horikoshii ?
-
?
additional information no activity with Pro, Asp or Glu Pyrococcus horikoshii OT-3 ?
-
?
additional information PH0138 gene encodes an amino acid racemase, AAR. No activity with L-Pro, D-Asp, and D-Glu Pyrococcus horikoshii OT-3 ?
-
?

Subunits

Subunits Comment Organism
? x * 52392, calculated from sequence Pyrococcus horikoshii
More peptide mass fingerprinting analysis Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
AAR
-
Pyrococcus horikoshii
PH0138
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
assay at Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pyrococcus horikoshii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Pyrococcus horikoshii
pyridoxal 5'-phosphate activity is dependent on. In the absence of pyridoxal 5'-phosphate (about 10%) the activity of the purified recombinant enzyme is still remaining and is fully restored by the addition of pyridoxal 5'-phosphate Pyrococcus horikoshii

Expression

Organism Comment Expression
Pyrococcus horikoshii activity is enhanced in cells grown in the medium supplemented with D-amino acids especially D-allo-Ile up

General Information

General Information Comment Organism
metabolism the enzyme is likely responsible for utilization of D-amino acids for growth Pyrococcus horikoshii
physiological function enzyme AAR is responsible for D-amino acid utilization in Pyrococcus horikoshii Pyrococcus horikoshii