Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | 1 mM, completely blocks activity | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52392 | - |
calculated from sequence | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Leu | Pyrococcus horikoshii | the enzyme is likely responsible for utilization of d-amino acids for growth | L-Leu | - |
? | |
D-Met | Pyrococcus horikoshii | the enzyme is likely responsible for utilization of d-amino acids for growth | L-Met | - |
? | |
D-Phe | Pyrococcus horikoshii | the enzyme is likely responsible for utilization of d-amino acids for growth | L-Phe | - |
? | |
D-Phe | Pyrococcus horikoshii OT-3 | the enzyme is likely responsible for utilization of d-amino acids for growth | L-Phe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | O57878 | - |
- |
Pyrococcus horikoshii | O57878 | PH0138 gene encodes an amino acid racemase, AAR | - |
Pyrococcus horikoshii OT-3 | O57878 | - |
- |
Pyrococcus horikoshii OT-3 | O57878 | PH0138 gene encodes an amino acid racemase, AAR | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
substrate: D-Ala, pH 8.0, 80°C | Pyrococcus horikoshii |
3.9 | - |
substrate: D-Val, pH 8.0, 80°C | Pyrococcus horikoshii |
10.5 | - |
substrate: D-Met, pH 8.0, 80°C | Pyrococcus horikoshii |
15.6 | - |
substrate: D-Leu, pH 8.0, 80°C | Pyrococcus horikoshii |
22.7 | - |
substrate: D-Phe, pH 8.0, 80°C | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine | - |
Pyrococcus horikoshii | L-alanine | - |
r | |
D-isoleucine | - |
Pyrococcus horikoshii | L-isoleucine | - |
r | |
D-Leu | broad substrate specificity | Pyrococcus horikoshii | L-Leu | - |
? | |
D-Leu | the enzyme is likely responsible for utilization of d-amino acids for growth | Pyrococcus horikoshii | L-Leu | - |
? | |
D-leucine | - |
Pyrococcus horikoshii | L-leucine | - |
r | |
D-Met | broad substrate specificity | Pyrococcus horikoshii | L-Met | - |
? | |
D-Met | the enzyme is likely responsible for utilization of d-amino acids for growth | Pyrococcus horikoshii | L-Met | - |
? | |
D-methionine | - |
Pyrococcus horikoshii | L-methionine | - |
r | |
D-Phe | the enzyme is likely responsible for utilization of d-amino acids for growth | Pyrococcus horikoshii | L-Phe | - |
? | |
D-Phe | Phe is the most preferred substrate among the amino acids, broad substrate specificity | Pyrococcus horikoshii | L-Phe | - |
? | |
D-Phe | the enzyme is likely responsible for utilization of d-amino acids for growth | Pyrococcus horikoshii OT-3 | L-Phe | - |
? | |
D-Phe | Phe is the most preferred substrate among the amino acids, broad substrate specificity | Pyrococcus horikoshii OT-3 | L-Phe | - |
? | |
D-phenylalanine | - |
Pyrococcus horikoshii | L-phenylalanine | - |
r | |
D-serine | - |
Pyrococcus horikoshii | L-serine | - |
r | |
D-tryptophan | - |
Pyrococcus horikoshii | L-tryptophan | - |
r | |
D-tyrosine | - |
Pyrococcus horikoshii | L-tyrosine | - |
r | |
D-valine | - |
Pyrococcus horikoshii | L-valine | - |
r | |
L-alanine | - |
Pyrococcus horikoshii | D-alanine | - |
r | |
L-leucine | - |
Pyrococcus horikoshii | D-leucine | - |
r | |
L-Methionine | - |
Pyrococcus horikoshii | D-Methionine | - |
r | |
L-Methionine | - |
Pyrococcus horikoshii OT-3 | D-Methionine | - |
r | |
L-phenylalanine | - |
Pyrococcus horikoshii | D-phenylalanine | - |
r | |
L-valine | - |
Pyrococcus horikoshii | D-valine | - |
r | |
additional information | no activity with Pro, Asp or Glu | Pyrococcus horikoshii | ? | - |
? | |
additional information | PH0138 gene encodes an amino acid racemase, AAR. No activity with L-Pro, D-Asp, and D-Glu | Pyrococcus horikoshii | ? | - |
? | |
additional information | no activity with Pro, Asp or Glu | Pyrococcus horikoshii OT-3 | ? | - |
? | |
additional information | PH0138 gene encodes an amino acid racemase, AAR. No activity with L-Pro, D-Asp, and D-Glu | Pyrococcus horikoshii OT-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52392, calculated from sequence | Pyrococcus horikoshii |
More | peptide mass fingerprinting analysis | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
AAR | - |
Pyrococcus horikoshii |
PH0138 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pyrococcus horikoshii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Pyrococcus horikoshii | |
pyridoxal 5'-phosphate | activity is dependent on. In the absence of pyridoxal 5'-phosphate (about 10%) the activity of the purified recombinant enzyme is still remaining and is fully restored by the addition of pyridoxal 5'-phosphate | Pyrococcus horikoshii |
Organism | Comment | Expression |
---|---|---|
Pyrococcus horikoshii | activity is enhanced in cells grown in the medium supplemented with D-amino acids especially D-allo-Ile | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is likely responsible for utilization of D-amino acids for growth | Pyrococcus horikoshii |
physiological function | enzyme AAR is responsible for D-amino acid utilization in Pyrococcus horikoshii | Pyrococcus horikoshii |