Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | the velocity of the D-Phe formation from the L-isomer is markedly affected by the concentration of DTT, whereas that of L-Phe formation from the D-isomer is less affected | Brevibacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
L-Phe | - |
Brevibacillus brevis | |
0.15 | - |
ATP | - |
Brevibacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
x * 95000, SDS-PAGE | Brevibacillus brevis |
100000 | - |
sucrose density gradient centrifugation | Brevibacillus brevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus brevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevibacillus brevis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-phenylalanine + H2O = AMP + diphosphate + D-phenylalanine | the amino group of Phe is essential for its binding to the aminoacyl adenylate reaction center. The carbonyl group is not at all or only weakly bound. The benzene ring of Phe which determines substrate recognition also seems to be of minor importance for substrate binding | Brevibacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Brevibacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Phe + H2O | - |
Brevibacillus brevis | AMP + diphosphate + D-Phe | - |
? | |
additional information | the enzyme catalyzes Phe racemization and activation. Catalyzes ATP-AMP exchange reaction, L-Phe dependent ATP-AMP exchange reaction | Brevibacillus brevis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 95000, SDS-PAGE | Brevibacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Brevibacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
formation of L-isomer from D-Phe | Brevibacillus brevis |
8.2 | 8.5 | formation of D-isomer from L-Phe | Brevibacillus brevis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the velocity of the D-Phe formation from the L-isomer is markedly affected by the concentration of DTT, whereas that of L-Phe formation from the D-isomer is less affected | Brevibacillus brevis |