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Literature summary for 5.1.1.15 extracted from

  • Ahmed, S.A.; Esaki, N.; Tanaka, H.; Soda, K.
    Properties of alpha-amino-epsilon-caprolactam racemase from Achromobacter obae (1983), Agric. Biol. Chem., 47, 1887-1893.
No PubMed abstract available
Search for more literature for 5.1.1.15

General Stability

General Stability Organism
freezing inactivates, particularly in dilute solution of less than 1 mg/ml Achromobacter obae

Inhibitors

Inhibitors Comment Organism Structure
2-Methyl-3-benzothiazolonehydrazone hydrochloride
-
 Achromobacter obae
Ala weak Achromobacter obae
CuSO4
-
 Achromobacter obae
D-cycloserine
-
 Achromobacter obae
epsilon-Caprolactam
-
 Achromobacter obae
Glu weak Achromobacter obae
Gly
-
 Achromobacter obae
HgCl2
-
 Achromobacter obae
hydroxylamine
-
 Achromobacter obae
L-His
-
 Achromobacter obae
L-Lys
-
 Achromobacter obae
L-Met
-
 Achromobacter obae
L-Orn
-
 Achromobacter obae
Leu weak Achromobacter obae
NEM
-
 Achromobacter obae
PCMB
-
 Achromobacter obae
phenylhydrazine
-
 Achromobacter obae
Pro weak Achromobacter obae
Sodium borohydride
-
 Achromobacter obae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
 L-alpha-amino-epsilon-caprolactam
-
 Achromobacter obae
8
-
 D-alpha-Amino-epsilon-caprolactam
-
 Achromobacter obae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Achromobacter obae 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 1 * 49000, SDS-PAGE Achromobacter obae
51000
-
 gel filtration Achromobacter obae

Organism

Organism UniProt Comment Textmining
Achromobacter obae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Achromobacter obae

Storage Stability

Storage Stability Organism
-20°C, 0.25 M sucrose, 10 mM potassium phosphate buffer, pH 7.3, 0.02 mM pyridoxal 5'-phosphate, 0.01% 2-mercaptoethanol, protein concentration: more than 0.3%, stable for at least 6 months Achromobacter obae
20°C, 0.25 M sucrose, enzyme retains approximately 90% of its initial activity after storage for 4 months Achromobacter obae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alpha-Amino-epsilon-caprolactam r Achromobacter obae D-alpha-Amino-epsilon-caprolactam
-
 ?
additional information the enzyme catalyzes alpha-proton exchange of the substrate with deuterium during racemization in deuterium oxide Achromobacter obae ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Achromobacter obae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 7.2, 20 min, stable Achromobacter obae
65
-
 pH 7.2, 20 min, 5% loss of activity Achromobacter obae
70
-
 pH 7.2, 20 min, 15% loss of activity Achromobacter obae
75
-
 pH 7.2, 20 min, 40% loss of activity Achromobacter obae
80
-
 pH 7.2, 20 min, 25% loss of activity Achromobacter obae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
 L-alpha-amino-epsilon-caprolactam Achromobacter obae
8.8
-
 D-alpha-amino-epsilon-caprolactam Achromobacter obae

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 at 25°C, 20 min, stable Achromobacter obae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate enzyme contains 1 mol of pyridoxal 5'-phosphate per mol of enzyme. Km: 0.00021 mM Achromobacter obae