Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.1.17 extracted from

  • Laiz, L.; Liras, P.; Castro, J.M.; Martin, J.F.
    Purification and characterization of the isopenicillin-N epimerase from Nocardia lactamdurans (1990), J. Gen. Microbiol., 136, 663-671.
No PubMed abstract available
Search for more literature for 5.1.1.17

General Stability

General Stability Organism
unstable enzyme, pyridoxal 5'-phosphate stabilizes Amycolatopsis lactamdurans

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate 0.1 mM, 36% inhibition Amycolatopsis lactamdurans
ammonium sulfate 100 mM, 47% inhibition Amycolatopsis lactamdurans
Cu2+ 1 mM, complete inhibition Amycolatopsis lactamdurans
Fe2+ 0.135 mM, complete inhibition Amycolatopsis lactamdurans
Hg2+ 1 mM, complete inhibition Amycolatopsis lactamdurans
I- 1 mM, 27% inhibition Amycolatopsis lactamdurans
iodoacetamide 0.1 mM; 40% inhibition Amycolatopsis lactamdurans
N-ethylmaleimide 0.1 mM; 33% inhibition Amycolatopsis lactamdurans
Zn2+ 1 mM, 50% inhibition Amycolatopsis lactamdurans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 isopenicillin N
-
 Amycolatopsis lactamdurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 gel filtration Amycolatopsis lactamdurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isopenicillin N Amycolatopsis lactamdurans part of the cephalosporin pathway penicillin N
-
 r

Organism

Organism UniProt Comment Textmining
Amycolatopsis lactamdurans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate, ammonium sulfate, Sephadex G-75, Mono Q Amycolatopsis lactamdurans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000727
-
-
 Amycolatopsis lactamdurans

Storage Stability

Storage Stability Organism
-70°C, 0.05 mM pyridoxal 5'-phosphate, 17 d, no loss of activity Amycolatopsis lactamdurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopenicillin
-
 Amycolatopsis lactamdurans penicillin N
-
 r
isopenicillin N part of the cephalosporin pathway Amycolatopsis lactamdurans penicillin N
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 59000, SDS-PAGE Amycolatopsis lactamdurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 slightly lower activity at 20 and 30°C Amycolatopsis lactamdurans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 45 reduced activity below 20°C or above 30°C Amycolatopsis lactamdurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 40 stable for 2 h Amycolatopsis lactamdurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Amycolatopsis lactamdurans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 strong decrease in activity above pH 8.0 and below pH 6.5 Amycolatopsis lactamdurans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9
-
 Amycolatopsis lactamdurans

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate stabilizes enzyme during purification, may be tightly bound to the enzyme Amycolatopsis lactamdurans