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Literature summary for 5.1.1.18 extracted from

  • Fujitani, Y.; Nakajima, N.; Ishihara, K.; Oikawa, T.; Ito, K.; Sugimoto, M.
    Molecular and biochemical characterization of a serine racemase from Arabidopsis thaliana (2006), Phytochemistry, 67, 668-674.
    View publication on PubMed
Search for more literature for 5.1.1.18

Activating Compound

Activating Compound Comment Organism Structure
ATP activates, synergy between Mg2+ and ATP on activity Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
 Arabidopsis thaliana
additional information no inhibition by EDTA of the racemase activity Arabidopsis thaliana
Ni2+
-
 Arabidopsis thaliana
Zn2+
-
 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of the bifunctional enzyme, overview Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates optimally 3.6fold at 1 mM Arabidopsis thaliana
Fe2+ activates 1.4fold at 1 mM Arabidopsis thaliana
Mg2+ activates 2.9fold at 1 mM Arabidopsis thaliana
Mn2+ activates 3.0fold at 1 mM, synergy between Mg2+ and ATP on activity Arabidopsis thaliana
additional information the enzyme requires divalent cations, effects of metal ions on the enzyme's dehydration activity, overview Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, about, recombinant enzyme, SDS-PAGE and amino acid sequence calculation Arabidopsis thaliana
60000
-
 recombinant enzyme, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine Arabidopsis thaliana
-
 D-serine
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q2PGG3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
additional information ubiquitous tissue expression of the enzyme Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine 13% of the activity with L-serine Arabidopsis thaliana D-alanine
-
 ?
L-arginine 7% of the activity with L-serine Arabidopsis thaliana D-arginine
-
 ?
L-glutamine 4.5% of the activity with L-serine Arabidopsis thaliana D-glutamine
-
 ?
L-serine
-
 Arabidopsis thaliana D-serine
-
 ?
L-serine highly specific for Arabidopsis thaliana D-serine
-
 ?
additional information the bifunctional enzyme catalyzes not only serine racemization but also dehydration of serine to pyruvate Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, about, recombinant enzyme, SDS-PAGE and amino acid sequence calculation Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
8 9.5 high activity within this range Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate required, bound through a Schiff base, conserved binding site structure involving Lys59, Phe58, Gly238, Ser314, and Asn86, overview Arabidopsis thaliana