Cloned (Comment) | Organism |
---|---|
gene lyr, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Proteus mirabilis |
Protein Variants | Comment | Organism |
---|---|---|
S394C | site-directed mutagenesis, the mutant shows 1.5fold increased activity with L-arginine compared to the wild-type enzyme | Proteus mirabilis |
S394N | site-directed mutagenesis, the mutant shows about 2.2fold increased activity with L-arginine compared to the wild-type enzyme | Proteus mirabilis |
S394T | site-directed mutagenesis, the mutant shows 1.8fold increased activity with L-arginine compared to the wild-type enzyme | Proteus mirabilis |
S394Y | site-directed mutagenesis, the mutant shows 2.1fold increased activity with L-arginine compared to the wild-type enzyme | Proteus mirabilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | completely inhibition of the enzyme's L-Lys racemization at 5 mM, partially restored by the addition of pyridoxal 5'-phosphate | Proteus mirabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 7 | L-arginine | pH 8.0, 50°C, recombinant mutant S394T | Proteus mirabilis | |
1 | 2.5 | L-arginine | pH 8.0, 50°C, recombinant mutant S394C | Proteus mirabilis | |
2 | 6 | L-lysine | pH 8.0, 50°C, recombinant mutant S394C | Proteus mirabilis | |
2 | 6 | L-lysine | pH 8.0, 50°C, recombinant mutant S394N | Proteus mirabilis | |
14.9 | - |
L-arginine | pH 8.0, 50°C, recombinant wild-type enzyme | Proteus mirabilis | |
17.1 | - |
L-arginine | pH 8.0, 50°C, recombinant mutant S394N | Proteus mirabilis | |
17.8 | - |
L-arginine | pH 8.0, 50°C, recombinant mutant S394Y | Proteus mirabilis | |
21.8 | - |
L-lysine | pH 8.0, 50°C, recombinant wild-type enzyme | Proteus mirabilis | |
25.2 | - |
L-lysine | pH 8.0, 50°C, recombinant mutant S394T | Proteus mirabilis | |
26.5 | - |
L-lysine | pH 8.0, 50°C, recombinant mutant S394Y | Proteus mirabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine | Proteus mirabilis | - |
D-lysine | - |
r | |
L-lysine | Proteus mirabilis BCRC10725 | - |
D-lysine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Proteus mirabilis | M4GGR9 | gene lyr | - |
Proteus mirabilis BCRC10725 | M4GGR9 | gene lyr | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) | Proteus mirabilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
568 | - |
purified recombinant His-tagged enzyme, substrate L-arginine, pH 8.0, 50°C | Proteus mirabilis |
887 | - |
purified recombinant His-tagged mutant S394Y, substrate L-lysine, pH 8.0, 50°C | Proteus mirabilis |
980 | - |
purified recombinant His-tagged mutant S394C, substrate L-arginine, pH 8.0, 50°C | Proteus mirabilis |
1043 | - |
purified recombinant His-tagged mutant S394T, substrate L-arginine, pH 8.0, 50°C | Proteus mirabilis |
1185 | - |
purified recombinant His-tagged mutant S394Y, substrate L-arginine, pH 8.0, 50°C | Proteus mirabilis |
1200 | - |
purified recombinant His-tagged mutant S394N, substrate L-arginine, pH 8.0, 50°C | Proteus mirabilis |
1633 | - |
purified recombinant His-tagged mutant S394N, substrate L-lysine, pH 8.0, 50°C | Proteus mirabilis |
2356 | - |
purified recombinant His-tagged mutant S394C, substrate L-lysine, pH 8.0, 50°C | Proteus mirabilis |
2828 | - |
purified recombinant His-tagged enzyme, substrate L-lysine, pH 8.0, 50°C | Proteus mirabilis |
3127 | - |
purified recombinant His-tagged mutant S394T, substrate L-lysine, pH 8.0, 50°C | Proteus mirabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine | - |
Proteus mirabilis | D-arginine | - |
r | |
L-arginine | - |
Proteus mirabilis BCRC10725 | D-arginine | - |
r | |
L-lysine | - |
Proteus mirabilis | D-lysine | - |
r | |
L-lysine | - |
Proteus mirabilis BCRC10725 | D-lysine | - |
r | |
additional information | enzyme residue S394 residue plays a crucial role in determining the preference of Lyr to lysine and arginine. No activity by wild-type and mutant enzyme with alanine, methionine, histidine, tryptophan, leucine, glutamine, asparagine and serine | Proteus mirabilis | ? | - |
? | |
additional information | enzyme residue S394 residue plays a crucial role in determining the preference of Lyr to lysine and arginine. No activity by wild-type and mutant enzyme with alanine, methionine, histidine, tryptophan, leucine, glutamine, asparagine and serine | Proteus mirabilis BCRC10725 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lyr | - |
Proteus mirabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant His-tagged enzyme, substrate L-lysine | Proteus mirabilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.83 | - |
L-arginine | pH 8.0, 50°C, recombinant wild-type enzyme | Proteus mirabilis | |
14.93 | - |
L-lysine | pH 8.0, 50°C, recombinant mutant S394Y | Proteus mirabilis | |
16.2 | - |
L-arginine | pH 8.0, 50°C, recombinant mutant S394C | Proteus mirabilis | |
17.45 | - |
L-arginine | pH 8.0, 50°C, recombinant mutant S394T | Proteus mirabilis | |
18.75 | - |
L-arginine | pH 8.0, 50°C, recombinant mutant S394N | Proteus mirabilis | |
19.67 | - |
L-arginine | pH 8.0, 50°C, recombinant mutant S394Y | Proteus mirabilis | |
31.25 | - |
L-lysine | pH 8.0, 50°C, recombinant mutant S394N | Proteus mirabilis | |
50.68 | - |
L-lysine | pH 8.0, 50°C, recombinant mutant S394C | Proteus mirabilis | |
55.43 | - |
L-lysine | pH 8.0, 50°C, recombinant wild-type enzyme | Proteus mirabilis | |
62.5 | - |
L-lysine | pH 8.0, 50°C, recombinant mutant S394T | Proteus mirabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | recombinant His-tagged enzyme, substrate L-lysine | Proteus mirabilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Proteus mirabilis |