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Literature summary for 5.1.2.2 extracted from

  • Hegeman, G.D.; Rosenberg, E.Y.; Kenyon, G.L.
    Mandelic acid racemase from Pseudomonas putida. Purification and properties of the enzyme (1970), Biochemistry, 9, 4029-4036.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
benzoate weak Pseudomonas putida
Diphenylacetic acid
-
Pseudomonas putida
DL-Atrolactate
-
Pseudomonas putida
DL-beta-Phenyllactate
-
Pseudomonas putida
iodoacetamide weak noncompetitive inhibitor Pseudomonas putida
NEM weak noncompetitive inhibitor Pseudomonas putida
p-chloromercuribenzoate weak noncompetitive inhibitor Pseudomonas putida
phenylacetate
-
Pseudomonas putida
Phenylmercaptoacetate
-
Pseudomonas putida
Phenyloxyacetate
-
Pseudomonas putida
phosphate
-
Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.093
-
D-mandelate
-
Pseudomonas putida
0.1
-
D(-)-mandelate 4-chloro-D-mandelate Pseudomonas putida
0.256
-
4-Bromo-D-mandelate
-
Pseudomonas putida
0.29
-
4-Hydroxy-D-mandelate
-
Pseudomonas putida
0.33
-
4-Methoxy-D-mandelate
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Divalent metal ions increase the affinity of the enzyme for the substrate. No absolute requirement Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mandelate Pseudomonas putida inducible enzyme L-mandelate
-
?
D-mandelate Pseudomonas putida A.3.12 inducible enzyme L-mandelate
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
A.3.12
-
Pseudomonas putida A.3.12
-
A.3.12
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
195
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-bromo-D-mandelate
-
Pseudomonas putida 4-bromo-L-mandelate
-
?
4-bromo-D-mandelate
-
Pseudomonas putida A.3.12 4-bromo-L-mandelate
-
?
4-chloro-D-mandelate
-
Pseudomonas putida 4-chloro-L-mandelate
-
?
4-chloro-D-mandelate
-
Pseudomonas putida A.3.12 4-chloro-L-mandelate
-
?
4-hydroxy-D-mandelate
-
Pseudomonas putida 4-hydroxy-L-mandelate
-
?
4-hydroxy-D-mandelate
-
Pseudomonas putida A.3.12 4-hydroxy-L-mandelate
-
?
4-methoxy-D-mandelate
-
Pseudomonas putida 4-methoxy-L-mandelate
-
?
D-mandelate
-
Pseudomonas putida L-mandelate
-
?
D-mandelate inducible enzyme Pseudomonas putida L-mandelate
-
?
D-mandelate
-
Pseudomonas putida A.3.12 L-mandelate
-
?
D-mandelate inducible enzyme Pseudomonas putida A.3.12 L-mandelate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Pseudomonas putida