Application | Comment | Organism |
---|---|---|
biotechnology | the demonstrated application of a membrane bioreactor will be a useful method for large-scale dynamic kinetic resulution (DKR) of mandelic acid and for possible other bioconversions in organic media | Pseudomonas putida |
Cloned (Comment) | Organism |
---|---|
expressed ind Escherichia coli | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.548 | - |
(S)-Mandelate | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-mandelate = (R)-mandelate | one pot enantioconvergent synthesis of R-mandelic acid ester from racemic mandelic acid is achieved by a novel aqueous/organic two-phase system with two-enzyme process consisting of (1) lipase-catalyzed enantioselective esterification of racemic mandelic acid in organic solvent and (2) in situ racemization of unreacted isomer, S-mandelic acid in aqueous phase by recombinant mandelate racemase | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-mandelate | - |
Pseudomonas putida | (R)-mandelate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mandelate racemase | - |
Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
650 | - |
(S)-Mandelate | - |
Pseudomonas putida |