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Literature summary for 5.1.2.2 extracted from

  • Choi, W.J.; Lee, K.Y.; Kang, S.H.; Lee, S.B.
    Biocatalytic enantioconvergent separation of racemic mandelic acid (2007), Sep. Purif. Technol., 53, 178-182.
No PubMed abstract available

Application

Application Comment Organism
biotechnology the demonstrated application of a membrane bioreactor will be a useful method for large-scale dynamic kinetic resulution (DKR) of mandelic acid and for possible other bioconversions in organic media Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expressed ind Escherichia coli Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.548
-
(S)-Mandelate
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-mandelate = (R)-mandelate one pot enantioconvergent synthesis of R-mandelic acid ester from racemic mandelic acid is achieved by a novel aqueous/organic two-phase system with two-enzyme process consisting of (1) lipase-catalyzed enantioselective esterification of racemic mandelic acid in organic solvent and (2) in situ racemization of unreacted isomer, S-mandelic acid in aqueous phase by recombinant mandelate racemase Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-mandelate
-
Pseudomonas putida (R)-mandelate
-
?

Synonyms

Synonyms Comment Organism
mandelate racemase
-
Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
650
-
(S)-Mandelate
-
Pseudomonas putida