KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.68 | - |
D-fructuronate | pH 8.5, 22°C | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYS1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructuronate | - |
Thermotoga maritima | D-tagaturonate | - |
r | |
D-tagaturonate | - |
Thermotoga maritima | D-fructuronate | - |
r | |
additional information | residue Asp161 likely interacts with the C3 hydroxyl group of D-TagA and deprotonates it. A divalent cation (likely Zn2+) chelated by His162 and possibly interacts with the C5 keto and C4 hydroxyl groups of D-TagA, thereby contributing to the cleavage of the adjacent C3-C4 bond. A carboxyl group of Glu266 then may promote the rotation and reprotonation of the C3 aldehyde group in the intermediate and the restoration of the broken C3-C4 bond to form D-FruA | Thermotoga maritima | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tm0440 | - |
Thermotoga maritima |
UxaE | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
40% of maximum activity | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
D-fructuronate | pH 8.5, 22°C | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
physiological function | expression of UxaE complements the Escherichia coli uxaB knockout mutant strain and restores growth on galacturonate. An inferred galacturonate to mannonate catabolic pathway in Thermotoga maritima can be reconstituted in vitro using a mixture of recombinant purified enzymes UxaE, UxaC and UxuB | Thermotoga maritima |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.1 | - |
D-fructuronate | pH 8.5, 22°C | Thermotoga maritima |