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Literature summary for 5.1.2.7 extracted from

  • Rodionova, I.A.; Scott, D.A.; Grishin, N.V.; Osterman, A.L.; Rodionov, D.A.
    Tagaturonate-fructuronate epimerase UxaE, a novel enzyme in the hexuronate catabolic network in Thermotoga maritima (2012), Environ. Microbiol., 14, 2920-2934.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.68
-
D-fructuronate pH 8.5, 22°C Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima Q9WYS1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructuronate
-
Thermotoga maritima D-tagaturonate
-
r
D-tagaturonate
-
Thermotoga maritima D-fructuronate
-
r
additional information residue Asp161 likely interacts with the C3 hydroxyl group of D-TagA and deprotonates it. A divalent cation (likely Zn2+) chelated by His162 and possibly interacts with the C5 keto and C4 hydroxyl groups of D-TagA, thereby contributing to the cleavage of the adjacent C3-C4 bond. A carboxyl group of Glu266 then may promote the rotation and reprotonation of the C3 aldehyde group in the intermediate and the restoration of the broken C3-C4 bond to form D-FruA Thermotoga maritima ?
-
?

Synonyms

Synonyms Comment Organism
Tm0440
-
Thermotoga maritima
UxaE
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
90
-
40% of maximum activity Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
D-fructuronate pH 8.5, 22°C Thermotoga maritima

General Information

General Information Comment Organism
physiological function expression of UxaE complements the Escherichia coli uxaB knockout mutant strain and restores growth on galacturonate. An inferred galacturonate to mannonate catabolic pathway in Thermotoga maritima can be reconstituted in vitro using a mixture of recombinant purified enzymes UxaE, UxaC and UxuB Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
9.1
-
D-fructuronate pH 8.5, 22°C Thermotoga maritima