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Literature summary for 5.1.3.2 extracted from
- UDP-galactose 4-epimerase from Kluyveromyces fragilis - catalytic sites of the homodimeric enzyme are functional and regulated (2009), FEBS J., 276, 6725-6740.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-UMP | a competitive, irreversible inhibitor, binds per dimer of epimerase as isolate and causes inactivation, transition profiles indicate the existence of a stable intermediate with one inhibitor-binding site remaining unoccupied. Reductive inhibition of this intermediate reduces the activity to 58% with modification of one catalytic site, negative cooperativity, inhibition mechanism, overview. Protective effect of 5'-UMP against modification of the arginine located at the catalytic site by 1,2-cyclohexanedione | Kluyveromyces marxianus |  |
| galactose | - |
Kluyveromyces marxianus | |
| glucose | - |
Kluyveromyces marxianus | |
| L-arabinose | reductive inhibition | Kluyveromyces marxianus | |
| UDP | - |
Kluyveromyces marxianus | |
| UTP | - |
Kluyveromyces marxianus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | biphasic Michaelis-Menten kinetics, kinetic patterns, overview. Michaelis-Menten relationship of the monomeric epimerase shows hyperbolic dependency | Kluyveromyces marxianus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose | Kluyveromyces marxianus | - |
UDP-alpha-D-galactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose | - |
Kluyveromyces marxianus | UDP-alpha-D-galactose | - |
r | |
| UDP-alpha-D-glucose | reaction mechanism modelling, overview | Kluyveromyces marxianus | UDP-alpha-D-galactose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | a homodimer, containing one catalytic site and one NAD+ as cofactor per subunit, not showing fast association-dissociation | Kluyveromyces marxianus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-galactose 4-epimerase | - |
Kluyveromyces marxianus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | one ligand bound per subunit | Kluyveromyces marxianus | |
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