Literature summary for 5.1.3.4 extracted from

Deupree, J.; Wood, W.A.
L-Ribulose-5-phosphate 4-epimerase from Aerobacter aerogenes (1975), Methods Enzymol., 41B, 412-419.

No PubMed abstract available

Search for more literature for 5.1.3.4

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Klebsiella aerogenes

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+	Klebsiella aerogenes

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	-	L-ribulose 5-phosphate	-	Klebsiella aerogenes

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+	Klebsiella aerogenes
Co2+	divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+	Klebsiella aerogenes
Mg2+	divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+	Klebsiella aerogenes
Mn2+	reactivated after EDTA treatment by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+	Klebsiella aerogenes
Ni2+	divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+	Klebsiella aerogenes
Zn2+	divalent metal ion required. Reactivated by addition of divalent metal ion in decreasing order: Mn2+, Co2+, Ni2+, Ca2+, Zn2+, Mg2+	Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
114000	-	high speed equilibrium analysis	Klebsiella aerogenes

Organism

Organism	UniProt	Comment	Textmining
Klebsiella aerogenes	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ribulose 5-phosphate	-	Klebsiella aerogenes	D-Xylulose 5-phosphate	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	9.5	-	Klebsiella aerogenes

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Release 2023.1 (January 2023)