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Literature summary for 5.1.99.5 extracted from

  • Pietzsch, M.; Syldatk, C.; Wagner, F.
    A new racemase for 5-monosubstituted hydantoins (1992), Ann. N. Y. Acad. Sci., 672, 478-483.
No PubMed abstract available
Search for more literature for 5.1.99.5

Activating Compound

Activating Compound Comment Organism Structure
cysteine slight stimulation Arthrobacter sp.
dithiothreitol up to 50% stimulation Arthrobacter sp.
EDTA slight stimulation Arthrobacter sp.
Sodium dodecyl sulfate slight stimulation Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 80% loss of activity Arthrobacter sp.
Hg2+ complete loss of activity Arthrobacter sp.
iodoacetamide complete loss of activity Arthrobacter sp.
p-mercuribenzoate complete loss of activity Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 4 * 21000, SDS-PAGE Arthrobacter sp.
84000
-
 gel filtration Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 strain DSM 3747
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter sp.

Reaction

Reaction Comment Organism Reaction ID
D-5-monosubstituted hydantoin = L-5-monosubstituted hydantoin keto-enol automerism of 5-monosubstituted hydantoins is responsible for racemization Arthrobacter sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5S)-5-(1H-indol-2-ylmethyl)-3-methylimidazolidine-2,4-dione 20.2% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5R)-5-(1H-indol-2-ylmethyl)-3-methylimidazolidine-2,4-dione
-
 r
(5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione
-
 Arthrobacter sp. (5R)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione
-
 r
(5S)-5-(2-methylpropyl)imidazolidine-2,4-dione 9.8% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5S)-5-(2-methylpropyl)imidazolidine-2,4-dione
-
 r
(5S)-5-(4-hydroxybenzyl)imidazolidine-2,4-dione 76.7% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5R)-5-(4-hydroxybenzyl)imidazolidine-2,4-dione
-
 r
(5S)-5-benzylimidazolidine-2,4-dione 62.7% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5R)-5-benzylimidazolidine-2,4-dione
-
 r
(5S)-5-[2-(methylsulfanyl)ethyl]imidazolidine-2,4-dione 20.4% of the activity with (5S)-5-(1H-indol-2-ylmethyl)imidazolidine-2,4-dione Arthrobacter sp. (5R)-5-[2-(methylsulfanyl)ethyl]imidazolidine-2,4-dione
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 21000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Arthrobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Arthrobacter sp. isoelectric focusing
-
 4.5