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Literature summary for 5.2.1.1 extracted from
- Gene cloning and characterization of maleate cis-trans-isomerase from Alcaligenes faecalis (1997), Biochem. Biophys. Res. Commun., 239, 74-79.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Alcaligenes faecalis |  |
| dithiothreitol | activates | Alcaligenes faecalis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Alcaligenes faecalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Alcaligenes faecalis | |
| iodoacetamide | - |
Alcaligenes faecalis | |
| N-Chlorosuccinimide | - |
Alcaligenes faecalis | |
| p-chloromercuriphenylsulfonate | - |
Alcaligenes faecalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
Maleate | - |
Alcaligenes faecalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27971 | - |
2 * 27971, calculation from nucleotide sequence | Alcaligenes faecalis |
| 28000 | - |
2 * 28000, SDS-PAGE | Alcaligenes faecalis |
| 60000 | - |
gel filtration | Alcaligenes faecalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alcaligenes faecalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alcaligenes faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Maleate | r, reverse reaction is quite weak | Alcaligenes faecalis | Fumarate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 28000, SDS-PAGE | Alcaligenes faecalis |
| dimer | 2 * 27971, calculation from nucleotide sequence | Alcaligenes faecalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | 45 | - |
Alcaligenes faecalis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | about 50% of maximal activity at 35°C and 50°C | Alcaligenes faecalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36.7 | - |
Maleate | - |
Alcaligenes faecalis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Alcaligenes faecalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.3 | about 50% of maximal activity at pH 6.5 and at pH 9.3 | Alcaligenes faecalis |
| 7 | 9 | more than 75% of maximal activity at pH 7.0 and pH 9.0 | Alcaligenes faecalis |
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