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Literature summary for 5.2.1.8 extracted from

  • Bailey, M.L.; Shilton, B.H.; Brandl, C.J.; Litchfield, D.W.
    The dual histidine motif in the active site of Pin1 has a structural rather than catalytic role (2008), Biochemistry, 47, 11481-11489.
    View publication on PubMed
Search for more literature for 5.2.1.8

Protein Variants

Protein Variants Comment Organism
H157A mutant supports viability in yeast complementation assay Homo sapiens
H157F mutant supports viability in yeast complementation assay Homo sapiens
H157L mutant supports viability in yeast complementation assay Homo sapiens
H157N mutant supports viability in yeast complementation assay Homo sapiens
H157S mutant supports viability in yeast complementation assay Homo sapiens
H59A mutant supports viability in yeast complementation assay Homo sapiens
H59F mutant supports viability in yeast complementation assay but displays significantly reduced growth in yeast compared to wild-type Pin1 Homo sapiens
H59L mutant is not viable Homo sapiens
H59L/H157A about 50% of wild-type activity with substrate Trp-Phe-Tyr-Ser(PO3H2)-(cis)-Pro-Arg-4-nitroanilide, no activity with substrate Ala-Glu-(cis)-Pro-Phe-4-nitroanilide Homo sapiens
H59L/H157F about 5% of wild-type activity with substrate Trp-Phe-Tyr-Ser(PO3H2)-(cis)-Pro-Arg-4-nitroanilide, 3% activity with substrate Ala-Glu-(cis)-Pro-Phe-4-nitroanilide Homo sapiens
H59L/H157L mutant supports viability in yeast complementation assay, mutation H157L rescues mutant H59L Homo sapiens
H59L/H157S about 5% of wild-type activity with substrate Trp-Phe-Tyr-Ser(PO3H2)-(cis)-Pro-Arg-4-nitroanilide, no activity with substrate Ala-Glu-(cis)-Pro-Phe-4-nitroanilide Homo sapiens
H59N mutant supports viability in yeast complementation assay Homo sapiens
H59S mutant supports viability in yeast complementation assay Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isoform Pin1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Glu-(cis)-Pro-Phe-4-nitroanilide
-
 Homo sapiens Ala-Glu-(trans)-Pro-Phe-4-nitroanilide
-
 ?
Trp-Phe-Tyr-Ser(PO3H2)-(cis)-Pro-Arg-4-nitroanilide
-
 Homo sapiens Trp-Phe-Tyr-Ser(PO3H2)-(trans)-Pro-Arg-4-nitroanilide
-
 ?