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Literature summary for 5.2.1.8 extracted from

  • Chen, J.; Chen, S.; Wang, J.; Zhang, M.; Gong, Z.; Wei, Y.; Li, L.; Zhang, Y.; Zhao, X.; Jiang, S.; Yu, L.
    Cyclophilin J is a novel peptidyl-prolyl isomerase and target for repressing the growth of hepatocellular carcinoma (2015), PLoS ONE, 10, e0127668 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.2.1.8

Application

Application Comment Organism
medicine CYPJ expression is upregulated in over 60% hepatocellular carcinoma tissues. CYPJ is found in the whole cell of hepatocellular carcinoma tissues with preferential location at the cell nucleus Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of CYPJ at 2.0 A and in complex with cyclosporin A. Residues His43, Arg44 and Gln52 are conserved active-site residues located in the shallow pocket, and Arg44 displayes flexible conformation Homo sapiens

Protein Variants

Protein Variants Comment Organism
K120A 87% of wild-type activity Homo sapiens
R44A 57% of wild-type activity Homo sapiens
R44A/F49A 32% of wild-type activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cyclosporin A
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.814
-
 N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide pH 8.0, 8.5°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information CYPJ is found in the whole cell of hepatocellular carcinoma tissues with preferential location at the cell nucleus Homo sapiens
-
-
nucleus CYPJ is found in the whole cell of hepatocellular carcinoma tissues with preferential location at the cell nucleus Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
hepatoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide
-
 Homo sapiens N-succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide
-
 r

Synonyms

Synonyms Comment Organism
cyclophilin J
-
 Homo sapiens
CyPJ
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
34.2
-
 N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide pH 8.0, 8.5°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.012
-
 pH 8.0, 8.5°C Homo sapiens cyclosporin A

Expression

Organism Comment Expression
Homo sapiens CYPJ expression is upregulated in over 60% hepatocellular carcinoma tissues up

General Information

General Information Comment Organism
physiological function CYPJ promotes the transition of cells from G1 phase to S phase by activating cyclin D1 promoter. CYPJ overexpression accelerates liver cell growth in vitro and in vivo. Inhibition of CYPJ by cyclosporin A or CYPJ-specific RNAi diminishes the growth of liver cancer cells in vitro and in vivo Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
42
-
 N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide pH 8.0, 8.5°C Homo sapiens