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Literature summary for 5.3.1.1 extracted from

  • Kurzok, H.G.; Feierabend, J.
    Comparison of a cytosolic and a chloroplast triosephosphate isomerase isoenzyme from rye leaves. I. Purification and catalytic properties (1984), Biochim. Biophys. Acta, 788, 214-221.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
AsO43-
-
Secale cereale
iodoacetate
-
Secale cereale
p-hydroxymercuribenzoate
-
Secale cereale
SO42- CuSO4 Secale cereale

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
D-glyceraldehyde 3-phosphate cytosolic enzyme Secale cereale
0.68
-
D-glyceraldehyde 3-phosphate chloroplastic enzyme Secale cereale
1.5
-
glycerone phosphate glycerone phosphate, cytosolic enzyme Secale cereale
2.5
-
glycerone phosphate chloroplastic enzyme Secale cereale

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Secale cereale 9507
-
cytosol
-
Secale cereale 5829
-

Organism

Organism UniProt Comment Textmining
Secale cereale
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Secale cereale

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Secale cereale
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glyceraldehyde 3-phosphate r Secale cereale Glycerone phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
33 40 cytosolic enzyme Secale cereale
50
-
chloroplastic enzyme Secale cereale

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 20°C: about 65% of maximal activity, 45°C: about 20% of maximal activity, cytosolic enzyme Secale cereale
35 53 35°C: about 25% of maximal activity, 53°C: about 70% of maximal activity, chloroplastic enzyme Secale cereale

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
5 min, cytosolic enzyme, about 50% of activity Secale cereale
55
-
5 min, chloroplastic enzyme, about 30% loss of activity Secale cereale

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.5 cytosolic enzyme Secale cereale
8.4
-
chloroplastic enzyme Secale cereale