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Literature summary for 5.3.1.1 extracted from

  • Borchert, T.V.; Pratt, K.; Zeelen, J.P.; Callens, M.; Noble, M.E.M.; Opperdoes, F.R.; Michels, P.A.M.; Wierenga, R.K.
    Overexpression of trypanosomal triosephosphate isomerase in Escherichia coli and characterisation of a dimer-interface mutant (1993), Eur. J. Biochem., 211, 703-710.
    View publication on PubMed
Search for more literature for 5.3.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
H47N mutant His47Asn, dimer is considerably less stable than wild-type trypanosomal enzyme Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 D-glyceraldehyde 3-phosphate recombinant wild-type enzyme Trypanosoma brucei
0.25
-
 D-glyceraldehyde 3-phosphate D-glyceraldehyde 3-phosphate Trypanosoma brucei
1.1
-
 glycerone phosphate recombinant wild-type enzyme Trypanosoma brucei
1.2
-
 glycerone phosphate
-
 Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2500
-
 recombinant wild-type enzyme Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Glyceraldehyde 3-phosphate r Trypanosoma brucei Glycerone phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1000
-
 glycerone phosphate recombinant enzyme Trypanosoma brucei
1080
-
 glycerone phosphate
-
 Trypanosoma brucei
6000
-
 D-glyceraldehyde 3-phosphate recombinant enzyme Trypanosoma brucei
6170
-
 D-glyceraldehyde 3-phosphate
-
 Trypanosoma brucei