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Literature summary for 5.3.1.1 extracted from
- Cloning, expression, purification and characterization of triosephosphate isomerase from Trypanosoma cruzi (1997), Eur. J. Biochem., 244, 700-705.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methyl methanethiosulfonate | the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana | Leishmania mexicana |  |
| methyl methanethiosulfonate | the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana | Trypanosoma brucei | |
| methyl methanethiosulfonate | the sensitivity of enzyme from Trypanosoma cruzi is about 40times higher than that of Trypanosoma brucei and 200times higher than that of Leishmania mexicana | Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
D-glyceraldehyde 3-phosphate | D-glyceraldehyde 3-phosphate | Trypanosoma brucei | |
| 0.9 | - |
D-glyceraldehyde 3-phosphate | - |
Trypanosoma cruzi | |
| 0.9 | - |
D-glyceraldehyde 3-phosphate | - |
Leishmania mexicana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27244 | - |
2 * 27244, calculation from nucleotide sequence | Trypanosoma cruzi |
| 50000 | - |
gel filtration | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania mexicana | - |
- |
- |
| Trypanosoma brucei | - |
- |
- |
| Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma cruzi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3527 | - |
- |
Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | Glycerone phosphate | - |
? | |
| D-Glyceraldehyde 3-phosphate | - |
Trypanosoma cruzi | Glycerone phosphate | - |
? | |
| D-Glyceraldehyde 3-phosphate | - |
Leishmania mexicana | Glycerone phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Trypanosoma brucei |
| dimer | - |
Leishmania mexicana |
| dimer | 2 * 27244, calculation from nucleotide sequence | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Trypanosoma brucei |
| 7 | 8.5 | - |
Trypanosoma cruzi |
| 7 | 8.5 | - |
Leishmania mexicana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity | Trypanosoma brucei |
| 6 | 9 | pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity | Trypanosoma cruzi |
| 6 | 9 | pH 6.0: about 60% of maximal activity, pH 9.0: about 50% of maximal activity | Leishmania mexicana |
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