Cloned (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
determination by molecular replacement, at 2.3 A resolution and in the closed state. Phosphate acts as a competitive inhibitor and occupies the binding pocket. Binding pocket has a very stable conformation even without a substrate | Helicobacter pylori |
Protein Variants | Comment | Organism |
---|---|---|
D213A | Residue putatively involved in a highly conserved salt bridge lacking in Helicobacter pylori enzyme. Kinetics and isomerization activity similar to wild-type | Helicobacter pylori |
D213Q | Residue putatively involved in a highly conserved salt bridge lacking in Helicobacter pylori enzyme. Kinetics and isomerization activity similar to wild-type | Helicobacter pylori |
K183A | Residue putatively involved in a highly conserved salt bridge lacking in Helicobacter pylori enzyme. Kinetics and isomerization activity similar to wild-type | Helicobacter pylori |
K183S | Residue putatively involved in a highly conserved salt bridge lacking in Helicobacter pylori enzyme. Kinetics and isomerization activity similar to wild-type | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | competitive, occupies the substrate binding pocket | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.49 | - |
D-glyceraldehyde 3-phosphate | mutant D213A, pH 7.5, 25°C | Helicobacter pylori | |
3.27 | - |
D-glyceraldehyde 3-phosphate | mutant D213Q, pH 7.5, 25°C | Helicobacter pylori | |
3.46 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Helicobacter pylori | |
3.56 | - |
D-glyceraldehyde 3-phosphate | mutant K183S, pH 7.5, 25°C | Helicobacter pylori | |
9.09 | - |
D-glyceraldehyde 3-phosphate | mutant K183A, pH 7.5, 25°C | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28108 | - |
2 * 28108, mass spectrometry | Helicobacter pylori |
56000 | - |
gel filtration | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | P56076 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Helicobacter pylori | dihydroxyacetone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28108, mass spectrometry | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1470 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.5, 25°C | Helicobacter pylori | |
1570 | - |
D-glyceraldehyde 3-phosphate | mutant D213A, pH 7.5, 25°C | Helicobacter pylori | |
1830 | - |
D-glyceraldehyde 3-phosphate | mutant D213Q, pH 7.5, 25°C | Helicobacter pylori | |
2000 | - |
D-glyceraldehyde 3-phosphate | mutant K183S, pH 7.5, 25°C | Helicobacter pylori | |
2330 | - |
D-glyceraldehyde 3-phosphate | mutant K183A, pH 7.5, 25°C | Helicobacter pylori |